摘要
为了探讨有机酸和氨基酸对双孢蘑菇2796菌丝生长的影响情况;此实验利用固体平板和液体摇瓶培养的方法,研究了柠檬酸、酒石酸、琥珀酸3种有机酸和丙氨酸、酪氨酸、精氨酸3种氨基酸在不同浓度下对双孢蘑菇菌丝生长的影响。通过测定各处理组的菌丝菌落直径、胞外蛋白质量比、菌丝干重、胞内多糖、过氧化物酶活性和还原糖质量比等生长参数;结果发现外源加入0.04%的柠檬酸最有利于双孢蘑菇的菌丝生长,可产生更多的菌丝胞内多糖、过氧化物酶、胞外蛋白。琥珀酸、精氨酸和酪氨酸也能够促进双孢蘑菇的生长,其中琥珀酸组的菌丝干重最高,比对照组增加了131%;精氨酸组的胞外蛋白质量比最高,比对照组增加了151%;柠檬酸组的胞内多糖质量比和过氧化物酶活性的值最高,分别比对照增加了21%和146%;添加酸组的还原糖质量比均比对照低。但添加过高浓度的有机酸和氨基酸,则抑制双孢蘑菇菌丝的生长。
The experiment objective was to study the effect of organic acid and amino acid on the mycelial growth of Agaricus Bisporus 2796;In the article,the effects of three kinds of organic acid from citric acid, tartaric acid and succinic acid and three kinds of amino acid from alanine,tyrosine and arginine in different concentration on the growth of Agaricus bisporus were studied by the solid and liquid culture methods.The growth parameters of the clony diameter,extracellular protein content,mycelium dry weight,intracellular polysaccharide content,peroxidase activity and reducing sugar content were analyzed in treat groups.The results showed that the growth of Agaricus bisporus could be mostly promoted by adding 0.04%citric acid.It would produce higher intracellular polysaccharide content,peroxidase activity and extracellular protein content.The mycelial growth could be also promoted by adding succinic acid,arginine and tyrosine.Compared to the control in the medium,the mycelium dry weight was highest by adding succinic acid,it increased 131%; the extracellular protein content was highest by adding arginine,it increased 151%;intracellular polysaccharide content and peroxidase activity of Agaricus bisporus were the highest value by adding citric acid,they increased 21%and 146%respectively.The reducing sugar content was declined by adding acids. But higher concentration organic acid and amino acid would inhibit the growth of Agaricus bisporus.
出处
《中国农学通报》
CSCD
北大核心
2010年第3期46-51,共6页
Chinese Agricultural Science Bulletin
基金
国家农业科技华东(江苏)创新中心--农业废弃物资源化工程技术研究中心开放课题(200801)
安徽师范大学大学生创新性实验计划项目资助(cxsy09010)
关键词
双孢蘑菇
菌丝
有机酸
氨基酸
发酵指标
Agaricus bisporus
mycelia
organic acid
amino acid
fermention indexes
