摘要
目的探讨大蒜新素对大鼠变应性鼻炎（allergic rhinitis，AR）模型血淋巴细胞中转录因子T-bet和GATA-3表达的影响。方法健康SD大鼠30只，雌雄各半，以随机数字表法分为3组，即药物组、对照组和空白组，每组10只。药物组用卵清蛋白（ovalbumin，OVA）辅以氢氧化铝和灭活百日咳杆菌佐剂建立AR模型，成功后每日腹腔注射大蒜新素0．4ml，剂量为25mg／（kg·d），连用10d。对照组造模方法同药物组，成功后每日腹腔注射生理盐水0．4ml，连用10d。空白组除无OVA致敏和激发外，其他处置方法同对照组。采集心脏血2ml，分离出血浆和淋巴细胞，采用酶联免疫吸附（ELISA）方法检测血浆中白细胞介素（interleukin，IL）4和1干扰素（interferon，IFN）的含量，采用反转录聚合酶链反应（RT—PCR）方法检测淋巴细胞中转录因子T—bet和GATA-3的表达，并应用半定量方法计算二者的相对表达量。结果药物组、对照组和空白组大鼠血浆中IL4的水平（x±s，以下同）分别是（6．292±1．734）、（14．252±1．971）、（4．916±0．600）pg／ml；IFN-γ的水平分别是（24．338±2．375）、（12．364±1．749）、（16．136±2．012）pg／ml。药物组血浆中IL4的水平低于对照组，差异有统计学意义（t=4．23，P〈0．05），与空白组比较，差异无统计学意义（t=1．01，P〉0．05）。药物组血浆中IFN-γ的水平高于对照组和空白组，差异有统计学意义（t值分别为4．61、5．79，P值均〈0．05）。对照组血浆中IFN-γ的水平和空白组比较，差异无统计学意义（t=1．17，P〉0．05）。药物组、对照组和空白组血淋巴细胞中转录因子GATA-3相对表达量的中位数（M）分别是0．826、1．029和0．474，3组间差异有统计学意义（χ2=6．599，P=0．019）；转录因子T—bet的相对表达量中位数（M）分别为1．245、0．280和0．544，3组间差异有统计�
Objective To investigate the effects of garlicin on the expression of transcription factors T-bet and GATA-3 in blood lymph ceUs of experimental rats with allergic rhinitis. Methods Thirty healthy SD rats were randomly divided into 3 groups, with 10 rats for each. Ten rats （pharmacological group） were sensitized and intranasally challenged by ovalbumin （OVA）, aluminium hydroxide hydrate gel and Bordetella pertussis （B. pertussis） inactive microorganism suspension adjuvants, as the allergic rhinitis models, and then injection of garlicin （0.4 ml） intraperitoneally per day for 10 days. Ten rats in the control group were immunized as the pharmacological group, and then injection of physiological saline as equal volume as garlicin. Ten rats in the negative control group were investigated using physiological saline only. Plasma and lymph cells were separated from 2 ml blood which was extracted from rat heart. Enzyme linked immunosorbent assay （ELISA） was utilized to detect the levels of plasma IL-d and IFN-γ, and RT-PCR was utilized to detect the expression of T-bet and GATA-3. Results The levels of IL-4 （x＋ s） were （6. 292 ＋ 1. 734）, （ 14. 252 ＋ 1. 971 ）, （4. 916 ＋ 0. 600 ） pg/ml, respectively, and the levels of IFN-γ were （24. 338＋2.375）, （12.364 ＋ 1.749）, （16.136 _＋ 2. 012） pg/ml, respectively, among the pharmacological, control and negative control groups. The plasma level of IL4 in the pharmacological group rats was lower than that of control group rats （ t = 4. 23, P 〈 0. 05 ）, while there was no significant difference between the pharmacological and negative control group rats （ t = 1.01, P 〉 0. 05 ）. The plasma level of IFN-γ was increased significantly in the pharmacological group rats when compared with the value of control group rats （ t = 4. 61, P 〈 0. 05 ） and with the negative control group （ t = 5.79, P 〈 0.05 ）, respectively. There was no significant difference （t = 1.17, P 〉 0.05） in plasma level of IFN-γ,
出处
《中华耳鼻咽喉头颈外科杂志》
CAS
CSCD
北大核心
2010年第2期133-138,共6页
Chinese Journal of Otorhinolaryngology Head and Neck Surgery
基金
广东省自然科学基金（8151008901000105）
“十一五”国家科技支撑计划（2007BAl07A26）作者单位：（陈冬、李添应、文卫平、史剑波）
昆明医学院附属第一医院耳鼻咽喉头颈外科（李玉晓、何晓光）通信作者：李玉晓,650031,Email：liyuxia07210@163.tom
