摘要
目的优化葎草多糖的超声辅助提取工艺,并研究其抑菌活性。方法以多糖中的葡萄糖含量为指标,对超声波功率、料液比和超声时间进行L9(34)正交实验,确定超声辅助提取葎草粗多糖的最佳工艺。用DE-52交换柱对葎草粗多糖进行分离,以滤纸片法研究葎草粗多糖和多糖分离组分的抑菌能力。结果以水为提取溶剂,料液比1∶28(g/ml),超声功率120W,超声90 min提取2次,葎草多糖的得率为0.755%。葎草粗多糖的抑菌效果具有一定的剂量依赖性;各多糖组分中HSP-Ⅰ、HSP-Ⅲ、HSP-Ⅳ发挥主要抑菌作用,其中HSP-Ⅰ效果最显著,HSP-Ⅱ抑菌效果不明显,且各组分间表现出协同关系。结论超声波辅助提取葎草多糖方法可行,得率高。葎草多糖具有抑菌活性。
Objective To optimize the ultrasound - assisted extraction method and to study the antibacterial activity of polysac-charides from Humulus scandens. Methods Taking the content of glucose as observation index, we optimized the extraction process by L9 （34）orthogonal experiment. The Humulus scandens polysaccharides （HSP） was purified into HSP -Ⅰ, HSP -Ⅱ, HSP - Ⅲ and HSP - Ⅳ by ion exchange chromatography with DE - 52. The antibacterial activity of each component was tested by means of filter paper. Results The optimal extraction conditions were obtained as follows ： water refluxing, a ratio of solid - liquid as 1 g-28ml, 120W for the intension of ultrasonic power, extracting time 90rain and extracting two times. Under aforesaid optimization conditions, the yield of polysaccharides was 0. 755%. Antibacterial experiments indicated that the inhibitory effect of crude polysaccharides showed dose - dependent. Among the isolated components, HSP - Ⅰ , HSP - Ⅲ, HSP - Ⅳ played main antibac- terial roles and HSP - Ⅱ was ineffective. It was cooperative with different isolated components. Conclusion The yield of Humulus Scandens polysaccharides is high with ultrasound - assisted extraction method. The Humulus scandens polysaccharides has obvious antibacterial action.
出处
《时珍国医国药》
CAS
CSCD
北大核心
2010年第2期342-344,共3页
Lishizhen Medicine and Materia Medica Research
基金
江苏省普通高校自然科学基金(No.06kjd180061)
关键词
葎草
多糖
分离
抑菌活性
Humulus scandens
Polysaccharides
Isolation
Antibacterial activity
