摘要
与固定化脂肪酶相比，游离脂肪酶具有反应速率快、成本较低的优势，成为制备生物柴油新的研究方向。前期研究结果表明，游离脂肪酶NS81006可以高效催化大豆油甲醇解制备生物柴油，进一步研究其对含酸油脂的催化，对于促进游离脂肪酶在生物柴油领域中的应用具有重要意义。本文系统研究了甲醇添加策略对游离脂肪酶NS81006催化油酸制备生物柴油的影响，进而考察了NS81006催化模拟酸化油以及实际含酸油脂制备生物柴油的转化情况。研究表明，在优化的甲醇添加策略下，游离脂肪酶NS81006可有效催化油酸、不同含酸量（0～100％，基于总质量）模拟酸化油以及实际含酸油脂进行生物柴油的制备；离心分离可有效实现NS81006的回复使用，连续回用5个批次，游离脂肪酶活性未出现明显下降。
Compared with immobilized lipase, soluble lipase has the advantages of faster reaction rate and lower cost, thus more and more attention has been paid to soluble lipase-mediated alcoholysis for biodiesel production. Our previous study showed that the soluble lipase NS81006 could catalyze the methanolysis of soybean oil for biodiesel production. Further exploration on NS81006-mediated alcoholysis of oils containing free fatty acid （FFA） would promote the application of soluble lipase in the field of biodiesel production. The influence of methanol adding strategy on NS81006-catalyzed methanolysis of oleic acid was studied firstly, and then, researches on NSS1006-mediated methanolysis of simulated acidified oil and different actual oil feedstocks containing FFA were carried out further. The results indicate that with the optimized methanol adding strategy, NS81006 could effectively catalyze the methanolysis of oleic acid, simulated acidified oil （0%-100%, based on total oil mass） and different oil feedstocks containing FFA for biodiesel production. Further study demonstrates that through centrifugation separation, NS81006 could be reused for biodiesel production and there is no obvious loss in lipase activity after its being reused for 5 batches.
出处
《高校化学工程学报》
EI
CAS
CSCD
北大核心
2010年第1期82-86,共5页
Journal of Chemical Engineering of Chinese Universities
基金
国家863高技术研究发展计划项目(2007AA100703)
清华大学基础研究基金(JC2007032)
国家自然科学基金(NSFC20706034)
关键词
生物柴油
游离脂肪酶
油酸
模拟酸化油
含酸油脂
biodiesel
soluble lipase
oleic acid
simulated acidified oil
oils containing free fatty acid
