摘要
目的用分光光度法测定角膜接触镜护理液中泊洛沙姆的含量,探讨护理液中其他润滑成分对测定的影响。方法选择市场上5种含有羟丙甲纤维素(HPMC)的多功能护理液,采用加热、迅速过滤的方法除去HPMC干扰后,采用以碘-碘化钾溶液为显色剂的分光光度法测定泊洛沙姆的含量。结果经过处理后的护理液分光光度法测定波长为500 nm,显色时间为120 min。泊洛沙姆的浓度范围在0.001～0.020 mg/mL内线性良好,标准曲线方程为Y=0.05091X+0.01715,r^2=0.9982。精密度实验(n=6)显示,精密度(RSD)为1.3314%,日间重复性RSD为2.9713%;回收率实验显示,80%、100%和120%的样品回收率为90%～105%。结论以碘-碘化钾溶液为显色剂的分光光度法操作简单、方法稳定且准确,可作为角膜接触镜护理液中有效成分泊洛沙姆质量控制的方法之一。
Objective To determine the contents of Poloxamer in contact lens care solution by spectrophotometric method, and investigate the effect of the other lubrication composition on the measurement. Methods Five brands of contact lens care solution with hydroxypropyl methyl cellulose （HPMC） were obtained. The interference of HPMC in contact lens care solution was removed by heating and rapid filtering, and the contents of Poloxamer was determined by KI-I2 speetropbotometry. Results For the pre-treated contact lens care solution, the tested wavelength was 500 nm and the development time was 120 rain. Favourable linearity was observed when Poloxamer ranged between 0. 001 mg/mL and 0.020 mg/mL, with standard eurve of Y = 0. 050 91X ＋ 0. 017 15 and r^2 = 0. 998 2. Precision （n = 6） test revealed that RSD was 1.331 4%, and RSD of daytime repeatability experiment was 2. 971 3%. The recovery rates of 80%, 100% and 120% samples ranged between 90% to 105%. Conclusion KI-I2 speetrophotometry is a convenient, stable and reliable method, and may serve as a mass control method for nonionic surfactant Poloxamer content in contact lens care solution.
出处
《上海交通大学学报（医学版）》
CAS
CSCD
北大核心
2010年第2期244-246,共3页
Journal of Shanghai Jiao tong University:Medical Science
