摘要
低离子强度和生理酸度下，藏红Ｔ（ＳＴ）与核酸的作用随ＳＴ与核酸的摩尔比（Ｒ）变化具有３种荧光特性，表现出３种结合机理．当Ｒ＞０３３，ＳＴ的荧光为核酸线性猝灭，最大激发波长发生紫移，但发射波长不变，是ＳＴ在核酸分子表面进行长距组装；Ｒ在０３３～０１３范围内，ＳＴ的荧光虽为核酸猝灭，但没有线性关系，表现为ＳＴ在核酸分子表面的组装与嵌入核酸碱基对结合处于微弱的平衡状态；当Ｒ＜０１３，ＤＮＡ体系产生新荧光，荧光激发峰红移，而发射波长仍保持不变，对应于ＳＴ嵌入核酸的双联碱基对之间，而ＲＮＡ体系却没有新的荧光产生．
At low ionic strength and physiological acidity, the interactions of safranine T(ST) with nucleic acids, depending on the molar ratio of ST to nucleic acids, show three kinds of fluorescence features which indicate three binding mechanism. When R is larger than 0 33, the fluorescence of ST was quenched with the excitation wavelength hypsochromicly shifting to 502 7nm and no variation of the emission wavelength, that is asscribed to the long range assembly of ST on the surface of nucleic acids. Although fluorescence is scarcely observed in the range of 0 33～0 13 of R , new fluorescence emission can be observed when R is lower than 0 13, that indicates the intercalative binding occurs for the interaction of ST with nucleic acids.
出处
《西南师范大学学报（自然科学版）》
CAS
CSCD
北大核心
1998年第6期672-676,共5页
Journal of Southwest China Normal University(Natural Science Edition)
基金
国家自然科学基金
重庆市中青年科技专家基金
西南师范大学自然科学基金
