摘要
目的:观察牡蛎肝宝对大鼠酒精性肝病(alcoholic liver disease,ALD)的防治作用及其抗脂质过氧化效应.方法:采用给予乙醇灌胃制造酒精肝模型的方法,75只♂Wistar大鼠随机均分为空白组、模型组以及牡蛎肝宝低剂量、中剂量、高剂量5组.模型组仅给予500mL/L的乙醇灌胃8wk,治疗组给予同等量乙醇灌胃,同时还给分别给予低剂量、中剂量和高剂量牡蛎肝宝8wk.空白对照组每天仅给予等量蒸馏水灌胃8wk.治疗结束后,抽取大鼠腹主动脉血液,检测血清ALT、AST、TG、TC、GLU及丙二醛加合物抗体IgG,同时取大鼠肝脏做冰冻切片,用苏丹Ⅲ染色,观察各组脂肪变程度.结果:空白组及牡蛎肝宝中、高剂量组血清ALT、AST、TG、TC、GLU均明显低于模型组(均P<0.01),牡蛎肝宝中、高剂量组血清丙二醛加合物抗体IgG浓度低于模型组(P<0.01),牡蛎肝宝低剂量组血清ALT、AST和GLU低于模型组(63.67U/L±6.89U/Lvs81.53U/L±22.85U/L,131.2U/L±11.63U/Lvs156.47U/L±32.23U/L,6.38mmol/L±0.66mmol/Lvs6.86mmol/L±0.67mmol/L,P<0.01或0.05);模型组肝脏病理改变的程度高于空白对照组(P<0.01);牡蛎肝宝中、高剂量组肝脏病理改变的程度均低于模型组(P<0.05).结论:牡蛎肝宝可以降低ALD的肝脏脂质蓄积,抗脂质过氧化,有保肝降酶作用.
AIM： To observe the protective and therapeutic effects and anti-lipid peroxidation effects of taurine ganbo in rats with alcoholic liver disease. METHODS： A rat model of alcoholic liver dis- ease was established by intragastric infusion of alcohol. Seventy-five male Wistar rats were randomly divided into five groups： normal control group, model group, low-dose taurine ganbo group, middle-dose taurine ganbo group, and high-dose taurine ganbo group. The model group was given dehydrated alcohol （500 mL/L） once a day for 8 weeks. The three taurine ganbo groups were treated as the model group and additionally given taurine ganbo once a day for 8 weeks. The normal control group was given equal volumes of distilled water for the same duration. After treatment, all the rats were killed. Serum alanine aminotransferase （ALT）, aspartate amino transferase （AST）, triglyceride （TG）, total cholesterol （T-CHO）, glucose （GLU）, and circulating IgG against malondialdehydemodified human serum albumin （MDA-HSA） were measured. Hepatic pathological changes were observed. RESULTS： The levels of serum ALT, AST, TG, T-CHO, and GLU were significantly higher in the model group than in the normal control group, the middle- and high-dose taurine ganbo groups （all P 〈 0.0）. The levels of serum ALT, AST and GLU in the low-dose taurine ganbo group were lower than those in the model group （63.67 U/L ± 6.89 U/L vs 81.53 U/L ± 22.85 U/L, 131.2 U/L ± 11.63 U/L vs 156.47 U/L ± 32.23 U/L, and 6.38 mmol/L ± 0.66 mmol/L vs 6.86 mmol/L ± 0.67 mmol/L, respectively; all P 〈 0.01 or 0.05）. The level of circulating IgG against MDA-HSA in the model group was higher than those in the normal control group and the middle- and high-dose taurine ganbo groups （all P 〈 0.01）. Compared to the normal control group, the model group had a much heavier lipid accumulation （P 〈 0.01）. The hepatic pathological changes were milder in the middle- and high-dose groups than in the model group （both P 〈 0.05�
出处
《世界华人消化杂志》
CAS
北大核心
2010年第4期340-345,共6页
World Chinese Journal of Digestology
关键词
牡蛎肝宝
酒精性肝病
乙醇
脂质过氧化
Taurine ganbo
Alcoholic liver disease
Alcohol
Lipid peroxidation
