摘要
目的:观察UO-126对人类宫颈癌细胞株HeLa细胞增殖及对F-盒和含蛋白7的WD重复结构域FBW7表达的影响。方法:采用MTT法观察不同浓度的UO-126对HeLa细胞增殖的影响;免疫荧光法检测FBW7在HeLa细胞中的定位和表达;RT-PCR、Western blot检测FBW7 mRNA及蛋白在UO-126阻断丝裂原活化蛋白激酶(mitogen-activated protein ki-nases,MAPK)信号通路前后的表达情况。结果:MTT结果显示各浓度UO-126具有抑制HeLa细胞增殖的作用,且具有剂量依赖性和时间依赖性(P<0.05)。免疫荧光检测显示UO-126处理HeLa细胞后FBW7阳性表达增强。RT-PCR及West-ern blot结果显示,UO-126作用后HeLa细胞FBW7 mRNA及蛋白表达水平较未处理HeLa细胞明显增高(P<0.05)。结论:MAPK信号通路阻断剂UO-126抑制HeLa细胞增殖,FBW7位于MAPK信号通路的下游。
AIM：To observe the effects of UO-126 on the expression of F-box and WD repeat domain-containing protein 7（FBW7）and on the proliferation of human Cervical cancer cell lines（HeLa cells）.METHODS：HeLa cells were treated with different concentrations of UO-126,MTT assay was used to observe the proliferation of HeLa cells.Immunofluorescence showed the lacation and expression of FBW7 in HeLa cells.The mRNA and protein expression of FBW7 were detected by RT-PCR and Western blot before and after mitogen-activated protein kinases（MAPK）signal was blocked by UO-126 a MAPK inhibitor.RESULTS：MTT results showed that the concentration range of MAPK signaling pathway inhibitor UO-126 inhibited the proliferation of HeLa cells in a concentration-and time-dependent manner（P〈0.05）.Immunofluorescence showed that the expression of positive FBW7 had increased after HeLa cells were treated with UO-126.RT-PCR and Western blot exhibited that the FBW7 mRNA and protein expression had significantly increased before and after HeLa cells were treated with UO-126（P〈0.05）.CONCLUSION：UO-126 could inhibit HeLa cells proliferation,FBW7 lied downstream of MAPK signaling pathway.
出处
《细胞与分子免疫学杂志》
CAS
CSCD
北大核心
2010年第2期138-140,144,共4页
Chinese Journal of Cellular and Molecular Immunology
基金
重庆市渝中区科委基金资助项目(2009)
关键词
UO-126
宫颈癌细胞株(HeLa细胞)
FBW7
MAPK
AIM：To observe the effects of UO-126 on the expression of F-box and WD repeat domain-containing protein 7（FBW7）and on the proliferation of human Cervical cancer cell lines（HeLa cells）.METHODS：HeLa cells were treated with different concentrations of UO-126,MTT assay was used to observe the proliferation of HeLa cells.Immunofluorescence showed the lacation and expression of FBW7 in HeLa cells.The mRNA and protein expression of FBW7 were detected by RT-PCR and Western blot before and after mitogen-activated protein kinases（MAPK）signal was blocked by UO-126 a MAPK inhibitor.RESULTS：MTT results showed that the concentration range of MAPK signaling pathway inhibitor UO-126 inhibited the proliferation of HeLa cells in a concentration-and time-dependent manner（P〈0.05）.Immunofluorescence showed that the expression of positive FBW7 had increased after HeLa cells were treated with UO-126.RT-PCR and Western blot exhibited that the FBW7 mRNA and protein expression had significantly increased bef...更多ore and after HeLa cells were treated with UO-126（P〈0.05）.CONCLUSION：UO-126 could inhibit HeLa cells proliferation,FBW7 lied downstream of MAPK signaling pathway. 还原
