摘要
目的:检测TNF-α、IFN-γ诱导HaCaT细胞β-防御素的表达。方法:用TNF-α、IFN-γ、TNF-α加IFN-γ干预HaCaT细胞后,RT-RCR方法检测hBD-2 mRNA、hBD-3 mRNA的表达。结果:TNF-α干预组HaCaT细胞中hBD-2 mRNA、hBD-3 mRNA,IFN-γ干预组HaCaT细胞中hBD-3 mRNA表达增高(P<0.05),干预12 h时的表达量高于干预24 h时的表达量;TNF-α与IFN-γ联合干预HaCaT细胞12 h时,hBD-2 mRNA、hBD-3 mRNA表达量最高。结论:TNF-α能刺激人角质形成细胞产生hBD-2和hRD-3,而IFN-γ仅能诱导产生hBD-3。
Objective： To detect the expression of human β - defensin - 2 （hBD - 2） mRNA, β - defensin - 3 （hBD - 3） mRNA induced by TNF- α, IFN - γ in human keratinocyte cell lines （HaCaT cells）. Methods： Cultured HaCaT cells were stimulated with TNF - α or IFN - γ, or a combination of TNF - α and IFN - γfor 12 h or for 24 h, respectively. The expression of hBD - 2 mRNA, hBD - 3 mRNA was detected by reverse transcription polymerase chain reaction. Results： The expression of both hBD - 2 mRNA and hBD - 3 mRNA was increased in HaCaT cells when induced by TNF - α, and only hBD - 3 mRNA expression was up - regulated when induced by IFN - γ. The expression was stronger at 12 h than at 24 h. 3he expression was strongest when induced by the combination of TNF - α and IFN- γ Conclusion： The expression of both hBD - 2 mRNA and hBD - 3 mRNA was increased in HaCaT cells when induced by TNF - α, and only hBD - 3 mRNA expression np - regulated when induced by IFN - γ.
出处
《中国麻风皮肤病杂志》
2010年第1期8-10,共3页
China Journal of Leprosy and Skin Diseases
基金
2008年山西医科大学科技创新基金(01200815)
