摘要
目的:建立并探讨基于连接酶反应检测硫嘌呤甲基转移酶(thiopurineS-methyltransferase,TPMT)基因分型分布的新方法。方法:建立多重PCR/连接酶检测方法检测TPMT基因多态性,并对50份健康志愿者样本进行检测。结果:本方法可靠、迅速,自动化程度高。研究未发现导致TPMT酶活性降低的基因型*2,*3A,*3B和*4,TPMT*3C仅发现2例杂合子。结论:本文建立的基因分型技术可为TPMT基因型检测提供可靠的方法。
AIM： To establish one ligase-based method to detect the gene polymorphism of TPMT. METHODS： The multiplex PCR/ligase detection reaction（L DR） was established, and 50 blood samples of healthy postulants were investigated with this method. RESULTS：This method was reliable, rapid, high-degree automatic. The genotype of ＊2, ＊3A, ＊3B and ＊4, which depressed the activity of TPMT were not found,only 2 samples of TPMT 3C heterozygote were detect- ed. CONCLUSION： This method can be applied for TPMT polymorphisms detection.
出处
《中国临床药理学与治疗学》
CAS
CSCD
2009年第11期1281-1285,共5页
Chinese Journal of Clinical Pharmacology and Therapeutics
基金
国家食品药品监督管理局药品评价中心国家科技支撑计划课题"安全用药关键技术与应用"课题编号(2006BI14B04)
关键词
硫嘌呤甲基转移酶
连接酶检测反应
硫唑嘌呤
遗传多态性
thiopurine S-methyltransferase
ligase detection reaction
azathiopurine
genetic polymorphism
