摘要
目的本研究旨在构建一种包含丙型肝炎病毒（HCV）保守区基因的新型DNA疫苗，并在小鼠模型中使用电转技术优化其免疫原性。方法首先，我们构建了包含HCV非结构蛋白NS3和核心蛋白Core部分基因序列的DNA疫苗，并证实了其表达；然后采用不同的体内电转方式于第0、4周分别免疫BALB／c小鼠，比较分析不同免疫方案的体液免疫（特异性IgG与抗体亚类）与细胞免疫应答（IFN-γ ELISPOT）的效果。结果使用电转技术可显著增强新型DNA疫苗免疫原性，采用皮内注射加卡钳电极电转的方式产生最强NS3特异性T细胞免疫反应。结论包含HCV保守区基因的新型DNA疫苗可通过优化电转技术增强免疫应答效果。这为我们下一步优化HCVDNA疫苗的免疫方案提供了依据。
Objective To characterize the immunogenicity in gene immunization of the conserved regions of hepatitis C virus（HCV） based on different delivery strategies. Methods We first constructed a novel DNA vaccine encoding a fusion gene（ from partial NS3 and Core） of HCV. Then we compared different protocols based on naked DNA injection twice or DNA injection with gene electrotransfer（GET） in BALB/c mice. The immune response was measured by antibody ELISA and by IFN-gamma ELISPOT. Results Our data showed that a protocol based on intradermally injection of DNA with optimal GET induced the strongest humoral and cellular immunity, and DNA with GET induced a substantially higher anti-NS3/Core T cell response than naked DNA injection. Conclusion Our data suggest that DNA vaccines encoding NS3/Core fusion protein of HCV immunized by the present strategy could merit further study in the context of future prophylactic and therapeutic HCV T cell based vaccines.
出处
《中华微生物学和免疫学杂志》
CAS
CSCD
北大核心
2010年第1期41-45,共5页
Chinese Journal of Microbiology and Immunology
基金
国家自然科学基金资助项目（30671961）
国家863课题资助项目（2007AA02Z455）
志谢：感谢美国NIH VRC的Dr.Nabel惠赠pVRC8301载体质粒.
关键词
丙型肝炎病毒
DNA疫苗
体内电转
Hepatitis C virus（HCV）
DNA vaccine
Intradermal electrotransfer
