摘要
目的评价纤维素对５氟尿嘧啶（５Ｆｕ）化疗后大鼠肠结构和屏障功能的影响。方法３０只Ｗｉｓｔａｒ大鼠随机分为饲料组（Ａ组）、肠内营养组（Ｂ组）和肠内营养加纤维素组（Ｃ组），每组１０只。Ｂ组和Ｃ组为等氮和等热卡。它们分别进食或接受肠内营养支持８天。术后第４天用５Ｆｕ化疗，第３和第７天分别测肠通透性（Ｌ／Ｍ），第８天测细菌移位、小肠和结肠的湿重、粘膜厚度、小肠的绒毛高度。实验前后分别测体重。结果Ｃ组体重丢失（－３１±３４ｇ）少于Ｂ组（－６６±５２ｇ）（Ｐ＜００５），而Ａ组体重增加（４９±４３ｇ），与Ｂ、Ｃ组比较差异有极显著意义（Ｐ＜００１）；Ｃ组的各项肠结构指标均优于Ｂ组（Ｐ＜００５）；化疗后Ｃ组和Ａ组的Ｌ／Ｍ变化无显著意义（Ｐ＞００５），而Ｂ组的Ｌ／Ｍ有显著意义的升高（Ｐ＜００１）；Ｃ组与Ａ组的细菌移位率（２０％）低于Ｂ组（７０％）（Ｐ＜００５）。
Objective To evaluate the effect of dietary fiber on intestinal structure and barrier function of 5 fruorouracil (5 Fu) challenged rat. Method Thirty Wistar rats were divided randomly into three groups (10 each group): Chow,enteric nutrition (EN), and EN+Fiber group. Both EN and EN+Fiber group were isonitrogenic and isocaloric. The rats kept their diet respectively for 8 days. 5 Fu was injected intraperitoneally on day 4 postoperatively. Intestinal permeability (L/M) was measured respectively on day 3 and day 7. On day 8, the bacterial translation, wet weight and mucousl thickness of both small intestine and colon and the villus height of small intestine were measured. The rats were weighted before and after experiment respectively. Result The body weight loss of the EN+Fiber group (-3.1±3 4 g) was less than that of the EN group (-6 6±5 2 g) ( P <0 05), whereas the Chow group gained body weight (4 9±4 3 g)( P <0 01 when compared with EN and EN+Fiber group). The parameters of intestinal structures of the EN+Fiber group was superior to the EN group ( P <0 05). The L/M of both EN+Fiber (from 0 026 5±0 007 3 to 0 027 4±0 006 8) and the Chow group (from 0 026 8±0 003 9 to 0 028 1±0 004 4) was unchanged ( P >0 05 for both) after 5 Fu challenged,whereas that of the EN group increased (from 0 028 9±0 007 0 to 0 033 1±0 008 4) ( P <0 01). The incidence of bacterial translocation to MLN of both EN+Fiber group and the Chow group (20%) was lower than that of the EN group (70%) ( P <0 05). Conclusion The dietary fiber protects the intestinal structure and barrier function of 5 Fu challenged rats.
出处
《中华外科杂志》
CAS
CSCD
北大核心
1998年第12期759-762,共4页
Chinese Journal of Surgery
