摘要
通过接种DF-1细胞（C/E）系,从山东某地方品系芦花鸡的鸡群中分离到一株外源性白血病病毒（ALV）SDAU09C2。与GenBank中已发表的不同亚群鸡ALV参考株的囊膜蛋白gp85的氨基酸序列比较,表明该分离株与B亚群ALV（ALV-B）2个参考株的gp85的氨基酸同源性最高,均为92.5%; 与A、C、D、E亚群ALV的gp85的氨基酸同源性仅在73.2%-87.9%之间; 而与J亚群gp85的氨基酸同源性更低至30.3%-32.4%。这是我国地方品系鸡群中第一次分离和鉴定ALV-B及其gp85基因的报道。
By inoculation of blood samples in DF-1 （C/E） cell cultlure, an exogenous avian leukosis virus （ALV） strain SDAU09C2 was isolated from a breeder farm of Chinese native breed ＂Luhua＂ in Shandong province. Comparisons of the amino acid sequence of env gene gp85 from the isolate with those from other ALV reference strains of different subgroups indicated that SDAU09C2 had the highest gp85 identity to two reference strains of subgroup B of 92.5%. Its gp85 identity to other chicken ALV subgroups A, C, D, E was in the range of 73.2%-87.9%. The identity to subgroup J was only 30. 3%-32.4%. This is the first report on isolation and identification of ALV-B and its gp85 from Chinese native breed chickens.
出处
《病毒学报》
CAS
CSCD
北大核心
2010年第1期53-57,共5页
Chinese Journal of Virology
基金
山东省2008~2009年农业重大应用技术创新课题
国家农业公益性行业科研专项经费项目(200803019)资助~~
关键词
B亚群禽白血病毒
GP85基因
芦花鸡
subgroup B avian leukosis virus
envelope gp85 gene
native Luhua breed
