摘要
概述了犬瘟热的细胞生物学与分子生物学诊断方法。在细胞生物学方面,对犬瘟热的诊断主要依靠病毒分离培养、动物回归试验、包涵体检查、病毒电镜形态检查、病毒特异性抗原及其抗体的检查等。随着分子生物学的发展,国内外相继建立起CDV核酸杂交诊断法、RT-PCR诊断法和real-timePCR诊断法。这些方法比传统诊断法具有更高的敏感性和特异性,尤其在犬瘟热的发病早期,机体尚未产生免疫应答时RT-PCR诊断法便能够作出早期诊断。
The development of diagnostic method of canine distemper（CD ） in cell biology and molecular biology was summarized in this paper. In the cell biology aspect, the diagnosis of CD was mainly depended on virus isolation and culture,artificial replication experiment, election microscope examination, inclusion body examination, specific antigen and antibody examination and so on. Along with the development of molecular biology, nucleic acid hybridization and RT-PCR diagnosis of canine distemper virus （CDV） have been established. These methods in sensitivity and specificity were higher compared with the traditional methods, especially in the earlier period of CDV infection when the animal body can＇t develop an immune response.
出处
《畜牧与饲料科学》
2009年第10期67-69,共3页
Animal Husbandry and Feed Science
