摘要
目的评价左氧氟沙星(喹诺酮类抗菌药)与磷霉素(合成抗菌药)联用对30株金黄色葡萄球菌的体外抗菌效应。方法用棋盘法设计,微量肉汤稀释法测定不同浓度组合的抗菌药物对15株甲氧西林敏感的金黄色葡萄球菌(MSSA)和15株甲氧西林耐药的金黄色葡萄球菌(MRSA)的最低抑菌浓度,并计算部分抑菌浓度指数(FICI)判定联合效应。结果左氧氟沙星与磷霉素联用后,其对金黄色葡萄球菌的MIC50显著降低,15株MSSA:FICI≤0.5占36.4%;0.5<FICI≤1占63.6%;1<FICI≤2为0;FICI>2为0。15株MRSA:FICI≤0.5占81.8%;0.5<FICI≤1占18.2%;1<FICI≤2为0;FICI>2为0。结论体外左氧氟沙星与磷霉素联用对30株金黄色葡萄球菌主要为协同和相加作用。
Objective To investigate the in vitro effects of levofloxacin combined with fosfomycin upon 30 strains of clinical isolates of Staphylococcus aureus （15 strains methicillin resistant and 15 strains methicillin sensitive Staphylococcus aureus ） . Methods A checkerboard method that adhered to the recommendations of the National Committee for Clinical Laboratory Standards was applied to assess the synergism effect between levofloxacin and fosfomycin. The FIC index was calculated according to the results. Results The MIC50 was reduced significantly for the combination of levofloxacin plus fosfomycin against Staphylococcus aureus. The FIC indexes of methicillin sensitive Staphylococcus aureus （MSSA） less than 0.5,from 0.5 to 1,from 1 to 2, more than 2 were 36.4% ,63.6% ,0,0 respectively. The FIC indexes of methicillin resistant Staphylococcus aureus （MRSA） less than 0. 5, from 0. 5 to 1, from 1 to 2, more than 2 were 81.8%, 18.2%, 0,0, respectively. Conclusion In vitro the combination of subinhibitory concentration of levofloxacin and fosfomycin presented synergistic and additive effect. There were no antagonism.
出处
《中国临床药理学杂志》
CAS
CSCD
北大核心
2009年第6期505-508,共4页
The Chinese Journal of Clinical Pharmacology
