摘要
背景与目的糖皮质激素如地塞米松能诱导淋巴细胞的凋亡并能防治因化疗引发的恶心及呕吐。然而,最近的研究资料显示糖皮质激素能引发欧洲人肺癌治疗耐药。中国人肺腺癌化疗时地塞米松对顺铂抑制肺腺癌原代细胞增殖效应的影响,目前尚不清楚。方法分离10例经病理学确诊为中国人源肺腺癌的原代细胞,经不同浓度的顺铂+/-地塞米松处理,采用四氮唑蓝比色法(MTT)检测肺腺癌原代细胞的增殖状况,并应用流式细胞仪检测顺铂联合地塞米松处理后的人肺腺癌细胞株A549的细胞凋亡率。结果在10例肺腺癌原代细胞中,地塞米松均可降低顺铂抑制肺腺癌原代细胞增殖的效应。同样,人肺腺癌细胞株A549,经顺铂联合地塞米松培养2周和3周后,地塞米松能降低癌细胞因顺铂诱导的细胞死亡率和凋亡率,提示地塞米松对顺铂诱导的细胞凋亡有抑制效应。结论地塞米松可以降低顺铂诱导肺腺癌细胞凋亡的作用,建议在肺腺癌化疗时谨慎使用地塞米松。
Background and objective Glucocorticoids, such as dexamethasone （DEX）, have been widely used in conjunction with cancer therapy due to inducing apoptosis in lymphoid cells and preventing nausea and vomiting. However, recent research indicates induction of therapy-resistance by glucocorticoids in lung cancer in European, although it is unclear whether DEX mediates the resistance of cisplatin-induced apoptosis in primary cells from resected Chinese human lung adeno- carcinoma specimens. Methods Ten primary cells from resected Chinese human lung adenocarcinoma specimens were treated with cisplatin in the presence or absence of DEX. The number of viable and dead cells was evaluated by trypan blue exclusion; the cell proliferation was measured by the MTT-assay; the cell apoptotic rate was analyzed by flow cytometry. Results In this study, it was found that DEX reduced the effect of cisplatin-induced proliferation inhibition in all of the primary cells from 10 examined tumor samples. On the other hand, the cell death and apoptosis induced by cisplatin in human lung adenocarcinoma ceils line A549 could also be inhibited in the presence of DEX during 2 and 3 weeks＇ co-incubation. Conclusion DEX may reduce the effect of chemotherapy with cisplatin, and it urges carefully reconsideration of the application of DEX in treatment of patients with lung adenocarcinoma in China.
出处
《中国肺癌杂志》
CAS
2010年第1期28-33,共6页
Chinese Journal of Lung Cancer
基金
浙江省自然科学基金(No.Y207802)
浙江省钱江人才资助基金(No.2007R10032)
嘉兴市科技计划项目基金(No.2007AY2034)资助~~
关键词
肺肿瘤
顺铂
地塞米松
Lung neoplasms
Cisplatin
Dexamethasone
