摘要
目的：为了明确贮脂细胞（ＦａｔＳｔｏｒｉｎｇＣｅｌｌ，ＦＳＣ）和肝细胞（Ｈｅｐａｔｏｃｙｔｅ，ＨＣ）在肝纤维化胶原合成中的作用。方法：本文在分离培养大鼠ＦＳＣ和ＨＣ的基础上，用＾３Ｈ－Ｐｒｏｌｉｎｅ掺入结合胶原酶消化法和定量斑点杂交法，分析了体外培养的ＦＳＣ和ＨＣ胶原和非胶原蛋白的合成以及Ｉ型前胶原ｍＲＮＡ的稳定性。结果：发现ＦＳＣ合成胶原蛋白的能力较ＨＣ强。
Aim: To understand the cellular sources of collagens in liver fibrosis. Methods: collagenase and pronase E were used to digest the rat liver, followed by Nycodenz density gradient centrifugation, fat storing cell(FSC)and hepatocyte(HC) were acquired,cultured and passaged in vitro.3H-proline incorporation combined with bacteria collagenase digestion were used to study the synthesis of collagen and non-collagen proteins in FSC and HC. Furthermore, actinomycin D was used to prevent the synthesis of cellular mRNA and proteins,based on this,semiquantitative Dot Blot Hybridization was used to study the stability of collagen mRNA type I . Results: It was found that FSC was more potent than HC in collagen synthesis (t-test, P< 0.05 ) ; opposite to this, less potent than HC in non-collagen protein. Furthermore, the collagen mRNA type I in FSC was more stable than that in HC, the half-life of the precollagen mRNA type I in FSC was longer than that in HC.Conclusion:These results indicate that FSC may play a leading role in the collagen synthesis of hepatic fibrosis,but the role of HC can not be ignored.
出处
《胃肠病学和肝病学杂志》
CAS
1998年第4期335-338,共4页
Chinese Journal of Gastroenterology and Hepatology
基金
国家自然科学基金资助项目
关键词
肝纤维化
肝细胞
胶原合成
胶原基因
基因表达
Hepatic fibrosis Hepatocyte Fat storing cell Collagen synthesis Collagen gene expression
