摘要
以旋果苣(Streptocarpus wendlanddii)的叶、花葶和花蕾为外植体,以MS为基本培养基,研究了组织培养快繁培养基中细胞分裂素和生长素最佳添加量和不同外植体的最佳灭菌时间。结果表明:在以1 g/L HgCl2溶液进行灭菌时,叶片的最佳灭菌时间是4～5 min,花葶、花蕾的最佳灭菌时间是4 min;在用"84"消毒液进行灭菌时,花葶、花蕾的最佳灭菌时间是5 min。愈伤组织诱导和根分化的最佳培养基均为MS+BA1.0 mg/L+NAA1.0 mg/L;芽分化的最佳培养基为MS+BA4.0 mg/L+NAA0.2 mg/L;继代培养最佳培养基为MS+BA1.0 mg/L+KT0.5 mg/L。
Leaf, scape and bud of Streptocarpus wendlanddii were used as explants in this study. Based on MS medium, the appropriate approach and sterilization time of tissue culture and rapid propagation of Streptocapus wendlanddii were obtained via regulating the concentrations of cytokinin and auxin. The resuits showed that when the explants were sterilized in 1 g/L mercuric chloride, the optimum sterilization time for leaf was 4--5 minutes, while bud was 4 minutes. When the explants were sterilized with 84 disinfectant liquid, the suitable time for scape and bud was 5 minutes. The optimal medium for callus in- duction was MS ＋ BA 1.0 mg/L ＋ NAA 1.0 mg/L;the optimal medium for bud differentiation was MS ＋ BA 4.0 mg/L＋ NAA 0.2 mg/L;the optimal medium for root differentiation was MS ＋ BA 1.0 mg/L ＋ NAA 1.0 mg/L;the optimal medium for subculture was MS ＋ BA 1.0 mg/L ＋ KT 0.5 mg/L.
出处
《吉林农业大学学报》
CAS
CSCD
北大核心
2010年第1期51-53,共3页
Journal of Jilin Agricultural University
基金
吉林省科技发展计划项目(20060224)
关键词
旋果苣
叶
花葶
花蕾
组织培养
Streptocarpus wendlandii
leaf
scape
bud
tissue culture
