摘要
目的:探讨荧光定量聚合酶链反应(FQ-PCR)技术用于孕妇外周血浆中游离胎儿DNA检测的可行性。方法:利用FQ-PCR技术特异性扩增Y基因性别决定区(SRY)并定量分析妊娠中期(18～25周)孕妇血浆中含量。结果:148例男胎孕妇血浆标本中,SRY基因检出率为98.65%。139例女胎孕妇血浆标本中未检测到SRY基因。结论:FQ-PCR技术在孕妇外周血游离胎儿DNA的检测中具有较高的灵敏性和特异性,FQ-PCR技术可应用于非创伤性产前诊断。
Objective： To explore the application value of quantitative fluorescent polymerase chain reaction （FQ- PCR） in the measurement of cell -free fetal DNA in the maternal circulation. Methods： The sex - determining region Y （SRY） gene of cell - free fetal DNA in the maternal （ gestational age of !8 to 25 weeks） circulation was detected with FQ - PCR. Results ：The SRY gene was detected in the 98.65% of the samples from 148 women who had the male fetuses. None of the SRY gene was detected in the samples from 139 women who had the female fetuses. Conclusion：The analysis of cell -free fetal DNA -SRY gene in the maternal circulation with FQ - PCR is highly sensitive and specific. It can be developed for non - invasive prenatal diagnosis.
出处
《中国计划生育学杂志》
北大核心
2010年第2期100-102,共3页
Chinese Journal of Family Planning
基金
山东省人口和计划生育科研发展项目(项目编号:2003-7)
关键词
胎儿DNA
荧光定量聚合酶链反应
非创伤性产前诊断
Fetal DNA
Quantitative fluorescent polymerase chain reaction
Non - invasive prenatal diagnosis
