摘要
本文借助GC/MS代谢组学方法,测定了育龄期妇女血清中酯化和非酯化脂肪酸轮廓谱,并对营养干预前后育龄期妇女血清脂肪酸含量变化进行了研究.按营养干预组(48例)和对照组(48例)分组,测定了育龄期妇女血清中酯化脂肪酸(EFAs)和非酯化脂肪酸(NEFAs)的平均含量;利用SIMCA-P软件对定量结果进行PLS-DA分析,聚类结果良好.基于定量结果,指认了6种潜在营养生物标志物,并由此初步探讨了这些潜在生物标志物的生理意义以及营养素干预对育龄期妇女血清脂肪酸含量的影响,以及脂肪酸代谢变化可能对胚胎神经发育造成的影响.
Nutrition intervention（NI） is the common means for Neural tube defects（NTDs） prevention. The serum EFAs and NEFAs metabolic profiling of Child-bearing age women was studied by GC/MS method. The subjects were random divided into two groups： NI group（ n = 48 ） and control group（ n = 48 ）. The mean levels of EFAs and NEFAs were reported. PLS-DA using the SIMCA-P software package allowed the different group to be distinguished, which means NI has great effects on the metabolism of fatty acids. Based on the quantification results, six FAs （both EFAs and NEFAs） were recognized as potential biomarkers. And the relationship between NI and the FAs＇ concentration variation was discussed to illustrate the potential impacts on the incidence of NTD.
出处
《高等学校化学学报》
SCIE
EI
CAS
CSCD
北大核心
2010年第1期46-50,共5页
Chemical Journal of Chinese Universities
基金
国家"九七三"计划项目(批准号:2007CB511903)资助
关键词
营养干预
神经管畸形
脂肪酸
营养代谢组学
生物标志物
Nutrition intervention
Neural tube defects （NTDs）
Fatty acid
Nutrition metabonomics
Biomarker
