摘要
目的体内观察雷帕霉素对食管鳞癌细胞生长及mTOR/4E-BP1信号通路的影响。方法给BALB/c裸鼠皮下注射食管鳞癌细胞株(EC9706),建立移植瘤模型,用雷帕霉素及顺铂对肿瘤进行治疗;用RT-PCR及Western blot等观察细胞形态及信号通路中各因子的变化。结果与对照组相比,雷帕霉素对肿瘤生长具有显著抑制作用(P<0.05),联合顺铂处理组的抑瘤作用更明显;雷帕霉素在体内降低了mTOR和p-4E-BP1的表达,并使4E-BP1的表达水平升高。结论雷帕霉素在体内能明显抑制食管鳞癌细胞EC9706裸鼠移植瘤的生长并抑制mTOR/4E-BP1信号通路的活性。
Objective To investigate the effects of rapamycin on tumor growth and mTOR/4E-BP1 signaling pathway in xenograft of esophageal squamous cell carcinoma （ESCC） EC9706 cells. Methods The expression of factors in mTOR/4E-BP1 signaling pathway of the tumor tissues was analyzed by RT-PCR and Western blot. Results Both rapamyein and cisplatin significantly inhibited the growth of transplantable tumors as compared to control group （P 〈 0. 05 ） and their combination had a synergestic effect. The results of RT-PCR and Western blot show that rapamyein significantly decreased the expression of mTOR and p-4E-BP1 but increased the expression of 4E-BP1. Conclusion Rapamycin inhibits the mTOR/4E-BP1 signalling pathway, resulting in the growth inhibition of xenograft of EC9706 cells.
出处
《基础医学与临床》
CSCD
北大核心
2010年第2期155-158,共4页
Basic and Clinical Medicine
