摘要
目的探讨系统性红斑狼疮（SLE）患者外周血单个核细胞（PBMC）中信号转导与转录激活因子2（STAT2）实时定量表达与SLE疾病特异性和病情活动度的相关性。方法收集144例SLE患者、27例非SLE患者与58名健康对照者的临床资料，取外周血抽提总RNA并逆转录成cDNA，运用SYBR green dyeⅠ实时定量聚合酶链反应（real—time PCR）法检测患者组和对照组的STAT2mRNA表达水平的差异，并与病情活动度进行分组比较，分析其意义。结果SLE患者组的STAT2定量表达（5．2±1．7）高于非SLE患者组和健康对照组（4．3±1．1，4．5±1．2，P均〈0．01）；SLE活动组的STAT2表达（5．2±1．5）高于非活动组（4．8±2．9，P〈0．01）；SLE患者组的STAT2表达与SLE疾病活动指数（SLEDAI）-2000和尿蛋白值之间呈正相关（r=0．317，0．309，P均〈0．01），与血清补体C3水平呈负相关（r=-0．449，P〈0．01）。结论转录因子STAT2在外周血细胞中的异常表达与SLE的发病机制有关，STAT2mRNA定量表达水平的升高与SLE患者病情活动相关。
Objective To investigate the expression of Signal transducer and activator of transcription 2 （STAT2） gene in the peripheral blood mononuelear cells of patients with systemic lupus erythematosus, and to evaluate the possible connections between STAT2 gene expression levels and clinical features. Methods One hundred and forty-four SLE patients, 27 non-SLE patients with other rheumatisms and 58 normal controls were recruited for this research, and the subjects were surveyed for clinical data collection. SYBR Green Dye based real-time quantitative PCR method was used to compare the expression levels of STAT2 in patients with SLE and those in the controls. The correlation of the gene expression levels and disease activity and specificity was studied. Results STAT2 expression levels （5.2±1.7） in SLE patients were remarkably higher than those in non-SLE patients and normal controls （4.3±1.1, 4.5±1.2, P〈0.01 in both）. The expression levels of STAT1 were increased in active SLE patients（5.2±1.5）, comparing with those observed in inactive SLE patients （4.8± 2.9, P〈0.01 ）, and expression levels of STAT1 in SLE patients were negatively correlated with C3 levels in sera （r=-0.449, P〈0.01 ） whereas were positively correlated with SLEDAI-2K score and 24 hour urine protein （r= 0.317, 0.309, P〈0.01 in both）. Conclusion Over-expression of STAT2 gene in the peripheral blood cells is linked with the pathogenesis of systemic lupus erythematosus, and the elevated expression level of STAT2 is correlated with SLE disease activity.
出处
《中华风湿病学杂志》
CAS
CSCD
北大核心
2010年第1期30-32,共3页
Chinese Journal of Rheumatology
基金
国家863计划基金（2002AA229101）
国家自然科学基金（30000154,30271224）
关键词
红斑狼疮
系统性
信号转导与转录激活因子2
Lupus erythematosus, systemic
Signal transducer and activator of transcription 2
