摘要
目的了解阴道壁成纤维细胞在应力情况下,Ⅰ型胶原(ColⅠ)、Ⅲ型胶原(ColⅢ)、脯氨酰羟化酶(PH4)及基质金属蛋白酶-2(MMP-2)基因表达水平的变化,探讨盆腔器官脱垂(POP)的发病机制。方法选取2008年11月至2009年3月于北京大学人民医院妇科接受手术治疗的女性POP患者阴道壁组织8例作为研究对象。以组织块法进行阴道壁成纤维细胞的原代培养,传代后进行细胞加载、加力,提取细胞RNA以测定ColⅠ、ColⅢ、PH4及MMP-2基因的表达水平。结果对于POP患者阴道壁成纤维细胞,不加力状态下,ColⅠmRNA的表达水平为0.666±0.287,而加力2圈状态下,ColⅠmRNA的表达水平为0.450±0.221,两者比较,差异接近有统计学意义(P=0.058)。而对于MMP-2mRNA的表达水平,不加力状态下为0.431±0.402,加力2圈状态下为0.167±0.099,两者比较,差异有统计学意义(P=0.039)。随着加力圈数的增加,其余各目的基因的表达水平均有下降趋势,但无统计学意义(P>0.05)。结论对于严重盆腔器官脱垂患者,在应力作用下,其盆底结缔组织中成纤维细胞的反应性降低。
Objective To detect the expression of type Ⅰcollagen （Col Ⅰ）,type Ⅲ collagen （Col Ⅲ）,prolyl-4-hydroxylases（PH4） and MMP-2 in fibroblasts obtained from patients with pelvic organ prolaspe （POP） using a specific equibiaxial strain system,thus to explore the possible mechanism of POP.Methods Eight patients with POP who underwent pelvic floor reconstructive surgery in Peking University People＇s Hospital from November 2008 to March 2009 were recruited in this study.Total RNA was extracted from the primary vaginal wall fibroblasts and gene expression of Col Ⅰ,Col Ⅲ,PH4 and MMP-2 were measured.Results The expression of Col Ⅰwas 0.666±0.287 when no strain applied,while it decreased to 0.450±0.221 under 2 cycles strain （P=0.058）. Expression of MMP-2 under strain was significant lower than that without strain （0.167±0.099 vs 0.431±0.402,P=0.039）. The expression of Col Ⅲ gene and PH4 gene were decreased along with the increaseing level of strain,but no significant difference was observed. Conclusions The fibroblasts of pelvic floor tissue in patients with POP may not be able to response to the strain applied due to the low reactivity.
出处
《中国妇产科临床杂志》
2010年第1期37-40,共4页
Chinese Journal of Clinical Obstetrics and Gynecology
基金
卫生部重点学科项目(2007)
首都医学发展科研基金项目(2007-301122)资助
