摘要
将草麻黄幼苗节间幼茎部分作外植体，培养在附加ＫＴ０．０５４ｍｇ／ｌ＋２，４－Ｄ１．１１ｍｇ／ｌ的ＭＳ培养基中，可成功诱导出愈伤组织，愈伤组织分化出芽；同样的外植体培养在附加ＢＡ３ｍｇ／ｌ＋ＩＡＡ０．２ｍｇ／ｌ的ＭＳ培养基中，诱导出大量不定芽；不同途径得到的芽转移到ＭＳ附加６－ＢＡ０．０５６ｍｇ／ｌ＋１．１１ｍｇ／ｌ２，４－Ｄ的培养基后，可分化出不定根，从而形成完整植株。
Callus was formed and buds were induced when internodal plants of Ephedra sinica Stampf were cultured on MS medium supplemented with 0.054mg/l KT and 1.11 mg/l 2,4-D. A lot of buds were induced when the same explants were cultured on MS medium supplemented with 3mg/l BA and 0.2mg/l IAA. Buds obtained from different paths were cultured on MS medium supplemented with 0.056mg/l 6-BA and 1.11mg/l 2,4-D, the adventitious roots differentiated and whole plantlets were formed.
出处
《中国草地》
CSCD
1998年第6期53-55,共3页
Grassland of China
基金
内蒙古自然科学基金
关键词
草麻黄
组织培养
愈伤组织
不定芽
植株再生
Ephedra sinica Stampf,\ Tissue culture,\ Callus,\ Adventitious bud,\ Adventitious root
