摘要
利用ＲＡＰＤ技术对松材线虫（Ｂｕｒｓａｐｈｅｌｅｎｃｈｕｓｘｙｌｏｐｈｉｌｕｓ）和拟松材线虫（Ｂ．ｍｕｃｒｏｎａ－ｔｕｓ）的分离物基因组ＤＮＡ进行了ＤＮＡ片段的随机扩增，以确定种间及种内群体的亲缘关系和筛选具有种鉴定特征的分子标记．通过对４０个１０聚体随机引物的筛选，应用８个引物对两种线虫种间及种内分离物扩增片段的遗传分析显示：两种间群体ＲＡＰＤ扩增片段的共享度为４０％～５６％，而在松材线虫的不同分离物之间的共享度为６０％～８４％；其中有一个引物（Ｐ２５３）可在松材线虫和拟松材线虫种间产生具有种鉴定特征的特异性ＲＡＰＤ片段，在松材线虫的不同分离物中均可产生一约７００ｂｐ的特异性片段，而在拟松材线虫中产生了两个分别约为１３００ｂｐ和１９００ｂｐ的特异性片段．
Two species in genera of Bursaphelenchus , B. xylophilus (Bx) and B. mucropatus (Bm), were examined for genetic relatedness among inter and infra species and screened for molecular marker with identical characteristics by random amplified polymorphic DNA (RAPD). Fourty ten mer random primers were screened and eight of which were used for genetic analysis. It is indicated that the similarity between inter species of Bx and Bm were 40%￣56%, and the similarity between isolates of infra species of Bx were 46%～84%. A tested primer (P253) showed discriminating RAPD fragments between Bx and Bm isolates, i.e. a fragments (ca. 700 bp) for all the three Bx isolates and two fragments (ca. 1 300 bp and 1 900 bp) for Bm isolates.
基金
中国博士后科学基金
关键词
松材线虫
拟松材线虫
RAPD标记
遗传分析
Bursaphelenchus xylophilus
B. mucronatus
RAPD marker
genetic analysis
