摘要
目的探讨镉恶性转化人支气管上皮细胞(16HBE)中各种翻译启动因子的表达变化。方法利用非转化16HBE对照细胞和氯化镉(CdCl2)恶性转化16HBE成瘤细胞,通过逆转录-聚合酶链反应(RT-PCR)及荧光定量PCR(FQ-PCR)等方法,观察与分析CdCl2恶性转化16HBE细胞中翻译启动因子家族mRNA的表达变化情况。结果以β-actin作为内参照,相对于对照细胞,在CdCl2转化16HBE成瘤细胞23个翻译启动因子的特异片段中,RT-PCR检测结果显示eIF2a、eIF2C1、eIF4E因子的mRNA表达水平均有不同程度的升高,而eIF2β、eIF4EBP因子的mRNA表达量则有不同程度的下降;FQ-PCR结果证实,镉恶性转化成瘤细胞中,eIF2a、eIF2C1、eIF4E的表达水平相对于对照细胞分别升高22倍、21倍和325倍,而eIF2β、eIF4EBP因子的表达量分别是对照细胞的0.005倍和0.107倍。结论研究结果提示,镉的致癌作用可能涉及到多个翻译因子的异常表达。
Objective To investigate expression changes of various translation initiation factors in human bronchial epithelial ceils （16HBE） induced by cadmium chloride. Methods The mRNA expressions of various translation initial factors in tumorigenic 16HBE cells induced by cadmium chloride （ CdCl2 ） were detected by semi-quantitative RT-PCR assay and measured by the fluorescent quantitative-polymerase chain reaction （FQ-PCR） respectively. Results With β-actin gene as an internal control,the primary results showed mRNA increased in eIF2a,eIF2Cl ,eIF4E and decreased in elF2β,eIF4EBP genes of CdCl2-tumorigenic 16HBE cells compared to the non-transformed cells. The results of FQ-PCR assay further indicated that the expressions of eIF2a,eIF2Cl ,eIF4E were elevated 22,21 and 325 times as compared to those of the controls,but the expressions of eIF2β and eIF4EBP were decreased apparently,only 01 005 and 0. 107 times as compared to the controls. Conclusion These results demonstrated that various translation initiation factors may be induced by cadmium carcinogensis.
出处
《中国职业医学》
CAS
北大核心
2009年第6期451-454,457,共5页
China Occupational Medicine
基金
国家自然科学基金资助项目(30771781)
广东省自然科学基金资助(06022672)
广州市市属高校科技计划项目(1002)
广州医学院自然科学基金重点项目(2006ZR004)
