摘要
用带盖离心管定量取转基因毛白杨组培苗及其室外8 a生植株叶片,加入DNA提取缓冲液研磨,离心后取上清液稀释5倍。取1μL作为PCR模板DNA检测杨树外源Bt和Npt II基因。PCR产物经琼脂糖凝胶电泳检测,目的条带清晰,可重复性强。
The leaves of tissue culture stocks and of 8 years old trees of transgenie Populus tomentosa were taken as materials using centrifuge tube. The materials were grinded for 15 - 20 s with DNA extraction buffer at room temperature. Samples were centrifuged,and the supematants of 5- fold diluted were used as a PCR template DNA to detect the Bt gene and the Npt II gene.
出处
《河北林果研究》
2009年第4期349-352,共4页
Hebei Journal of Forestry and Orchard Research
基金
河北省科技厅资助项目(03547016D)
河北省林业局资助项目(0207216)
关键词
转基因毛白杨
模板DNA制备
PCR检测
transgenic Populus tomentosa
preparation of template DNA
PCR detection
