摘要
为掌握规模化猪场猪群血清猪瘟抗体水平的消长情况,采用阻断ELISA检测方法对广西12个大中型规模化猪场的639份血清样品进行猪瘟抗体检测。结果表明,所测样品中抗体阳性率为90.14%,阴性率为6.26%,可疑率为3.60%,抗体阻断平均值为70.54,抗体差异度为19.93%;其中母猪的阳性率最高,达93.41%,商品猪阳性率最低,仅为86.50%。文章还分析了免疫失败的原因,并结合实际情况,提出了淘汰免疫缺陷种猪、准确注射、加强疫苗运输及保存冷链建设、购买优质疫苗、加强疫苗接种与免疫监测的有机结合等建议,以期为规模化猪场在选择疫苗及制定免疫程序时提供参考。
To investigate the changes in antibody of classical swine fever（CSF） in serum of swine population,639serum samples were collected from 12 middle and large sized pig farms in Guangxi and analyzed using ELISA method.The results showed 90.14% positive rate for CSF antibody amongst all samples,while the negative and suspected rates were 6.26%,and 3.60%,respectively.The mean value of antibody inhibition was found to be 70.54 with the 19.93% variance ratio of antibody.Among all the samples,the highest positive rate was recorded in sows（93.41%）,while it was the lowest in the commercial pig serum samples（86.50%）.Further,the causes of failure in immunity have also been analyzed in the present study,a number of control measures,viz.,eliminating immuno-deficient pigs in breeding,accurate rejection,efficient vaccine transportation and preservation chain construction,purchasing better vaccines,enhancing the inoculation and immunity determination of vaccine have been suggested to provide information on selecting vaccine and effectively running the immunity programs.
出处
《广西农业科学》
CSCD
2009年第12期1615-1617,共3页
Guangxi Agricultural Sciences
基金
南宁市科技支撑项目(2006204124)
关键词
猪瘟
抗体检测
免疫效果
ELISA
广西
classical swine fever antibody detection immunity effect ELISA Guangxi
