摘要
目的探讨p16基因甲基化在乳腺癌发生、发展中的作用。方法应用甲基化特异性PCR(MSP)联合测序检测58份乳腺癌及其癌旁组织中p16基因甲基化状态,应用免疫组化SP法检测p16及ER蛋白表达情况,对各指标间关系行Spearm an相关分析。结果p16基因在乳腺癌中甲基化率为29.3%(17/58),有淋巴结及远处转移者显著高于无转移者(P<0.05);p16甲基化及非甲基化者p16蛋白表达阻性(失表达)率分别为82.4%(14/17)、43.9%(18/41),ER蛋白失表达率分别为76.5%(13/17)、24.4%(10/41),P均<0.05。结论p16基因甲基化在乳腺癌发生、发展中具有重要作用,机制可能与调节p16和ER蛋白表达有关。
Objective To investigate the roles of p16 gene methylation in the occurrence and development of breast cancer. Methods Methylation-specific PCR（MSP） and DNA sequencing were used to analyze the methylation status of p16 gene in 58 breast cancers and adjacent normal tissues. Immunohistochemistry was used to investigate the expression of p16 and estrogen receptor（ER） protein. Spearman analysis was used to study the correlations between the indicators. Results Among the 58 breast eancers,pl6 hypermethylation was detected in 17 cases（29.3% ）, and which was more frequent in individuals with lymph node metastasis （ P 〈 0.05 ）. Loss expression rate of p16 protein in the methylated cases and unmethylated caseswere 82.4% （ 14/17 ） and 43.9% （ 18/41 ）, while the loss expression rate of ER were 76.5 % （13/17） and 24.4% （ 10/41 ）, both P 〈 0.05. Conclusions p16 gene methylation play an important role in the carcinogenesis and proggression of breast cancer,the mechanisms may be related to the regulation of p16 and ER protein expression.
出处
《山东医药》
CAS
北大核心
2010年第3期23-25,共3页
Shandong Medical Journal
基金
河北省科学委员会计划资助项目(0627611089)
