摘要
建立了香连丸及组方药材中药根碱、巴马汀、小檗碱、木香烃内酯和去氢木香内酯的含量测定方法．采用反相高效液相色谱，Hypersil ODS（5μm，4．6mm×250mm）色谱柱，流动相为乙腈：水（70：30，V：V），流速1．0mL／min，柱温30℃，检测波长为210n／n测定木香烃内酯和去氢木香内酯的含量；采用高效液相胶束色谱，Kromasil ODS（5la,m，4．6mm×150mm）色谱柱，流动相为0．2mol／LNaH2P04水溶液：7．00mmol／L十二烷基硫酸钠：乙腈（35：35：30，V：V：V），流速1．0mL／min，柱温30℃，检测波长为350nm测定药根碱、巴马汀、小檗碱的含量．结果显示5种活性成分在适当的线性范围内均具有良好的线性关系（r大于0．9993），平均回收率在87．67％～102．37％之间，RSD小于3．13％；结果表明方法简便、灵敏、准确，重现性好．
Based on the determination of contents of active components in Xianglianwan pills and comprised medicinal materiinals, an available and practical method for controlling the quality of preparation and medicinal materials has been set up. The contents of costanolide and dehydrocostuslactone were assayed under chromatographic conditions as follows： Hypersil ODS column （5 μm, 4.6 mm × 250 mm）, 30 ℃, mobile phase of acetonitrile and water （70： 30, V/V） at a flow rate of 1.0 mL/min, and the detective wavelength of 210 nm. Jatrorrhizine, palmatine, berberine in Xianglianwan pills and corresponding comprised medicinal materials were simultaneously determined under the following conditions： Kromasil ODS （5 μm, 4.6 mm ×150 mm）, the column temperature 30 %, phase flow rate of 1.0 mL/min, and detective wavelength, 350 run. All the compounds showed good linearity （r 〉 0. 9993） in the range of test concentrations, average recoveries of the method were between 87.67% and 102.37%, RSD 〈 3. 13%, the results showed that this method is simple, sensitive, accurate and reproducible.
出处
《分析测试技术与仪器》
CAS
2009年第4期220-225,共6页
Analysis and Testing Technology and Instruments
关键词
香连丸
含量测定
质量控制
高效液相色谱
Xianglianwan pills
content determination
quality control
HPLC
