摘要
目的:探讨乳腺癌17号染色体多体对人类表皮生长因子受体2(epidermal growth factor receptor 2,HER2)检测结果的影响及其临床病理学意义。方法:采用荧光原位杂交(fluorescencein situhybridization,FISH)双色法检测71例原发性浸润性乳腺癌中HER2基因和17号染色体拷贝数目情况。采用HER2绝对拷贝数标准和HER2/chromosome 17比值标准,判定HER2的FISH检测结果;基于FISH检测结果,结合免疫组织化学法(immunohistochemistry,IHC)检测的HER2蛋白表达情况及相关临床病理参数进行分组对比分析。结果:无论按照HER2绝对拷贝数标准(14/71,19.7%)或HER2/chromosome17比值标准(2/71,2.8%),所有FISH检测结果示HER2基因扩展可疑的病例都为17号染色体多体;与HER2基因扩增阴性者相比,单纯17号染色体多体组在肿瘤分级、淋巴结转移以及激素受体表达上差异无统计学意义(P均>0.05);而与HER2基因扩增阳性组相比,单纯17号染色体多体组表现出更低的肿瘤分级(50.0%vs81.5%,P=0.025)、更高的淋巴结阴性率(55.6%vs25.9%,P=0.045)以及更高的雌激素受体(estrogen receptor,ER)阳性率(83.3%vs41.7%,P=0.005)和孕激素受体(progestogen receptor,PR)阳性率(87.5%vs44.4%,P=0.003)。结论:相比于HER2基因扩增组,单纯17号染色体多体组更倾向于HER2基因扩增阴性;17号染色体多体会影响HER2检测结果,是FISH检测中导致可疑结果产生的主要原因。
Objective：To investigate the impact of polysomy 17 of breast cancer on testing results of human epidermal growth factor receptor 2（HER2） and its clinicopathologic significance.Methods：Seventy-one patients with primary invasive breast carcinoma were studied.The HER2 gene and chromosome 17 copy numbers were determined by dual-color fluorescence in situ hybridization（FISH）.The testing results were expressed by ABSolute HER2 gene copy number or the ratio of HER2 to chromosome 17.Based on the FISH testing results and HER2 protein expression determined by immunohistochemistry the results were compared between different groups divided by related clinicopathologic parameters.Results：All patients who had doubtable FISH results,either by ABSolute HER2 copy number（14 of 71 patients;19.7%） or by the ratio HER2/chromosome 17（2 of 71 patients,2.8%）,displayed polysomy 17.Polysomy 17-positive patients had no significant difference with HER2-negative patients in tumor grade,lymph node metastasis,and estrogen receptor（ER） expression（all P0.05）;but compared with HER2-positive patients,they showed lower tumor grade（50.0% vs 81.5%,P=0.025）,higher rate of negative lymph node（55.6% vs 25.9%,P=0.045）,and higher rate of ER positive expression（83.3% vs 41.7%,P=0.005） and progesterone receptor（PR）positive expression（87.5% vs 44.4%,P=0.003）.Conclusion：Compared with HER2 gene amplification group,polysomy 17-positive group tends to have negative HER2 gene expression.Polysomy 17 influences the testing results of HER2 and may be the main factor that caused doubtable results in FISH examination.
出处
《肿瘤》
CAS
CSCD
北大核心
2010年第1期36-41,共6页
Tumor
基金
卫生部科研基金资助项目(编号:WKJ2007-3-001)
关键词
乳腺肿瘤
染色体
人
17对
原位杂交
荧光
基因
ERBB-2
染色体多体
Breast neoplasms
Chromosomes
human
pair 17
In situ hybridization
fluorescence
Genes
erbB-2
Chromosome polysomy
