摘要
目的观察弓形虫感染妊娠中期的BALB/c小鼠后,对胎盘组织中细胞凋亡相关蛋白Bax、Bcl-2、Fas、FasL及TNF-α表达的影响,探讨弓形虫感染导致细胞凋亡增加的可能机制。方法在妊娠第8d,实验组经腹腔无菌接种纯化的弓形虫速殖子100个,溶于0.2mL PBS;对照组注射等量无菌PBS(0.01mol/L,pH=7.4)。于妊娠第12、14、16、18d,随机处死实验组和对照组孕鼠各5只,采用免疫组织化学法(i mmunohistochemistry)检测胎盘组织中细胞凋亡相关蛋白Bax、Bcl-2、Fas、FasL及TNF-α的表达水平。结果免疫组化显示,细胞凋亡相关蛋白在胎盘绒毛和蜕膜组织中均有表达,以胎盘合体滋养细胞表达较多。Bax、Fas、FasL及TNF-α的表达量实验组和对照组均随着妊娠期的延长而增加,相同妊娠天数实验组的表达量均比对照组多。Bcl-2的表达量均随着妊娠期的延长而减少,相同妊娠天数实验组的表达量均比对照组少。结论弓形虫感染妊娠中期的小鼠,可引起胎盘组织中细胞凋亡相关蛋白表达量的改变,进而启动内、外源性的细胞凋亡途径,导致胎盘细胞凋亡增加。
To observe the influence of the placental apoptosis on the expression of Bax,Bcl-2,Fas,FasLand TNF-α during the second trimester of pregnancy,mice of experimental group were intraperitoneal injected with 100 purified Toxoplasma gondii tachyzoites added in 0.2mL of PBS,while those of the control group were injected with 0.2 mL of sterile PBS（0.01 mol/L,pH 7.4）in the 8-th day of pregnancy.During the 12,14,16 and 18-th days of pregnancy,5 mice both in experimental and control group were randomly killed and the expression levels of the apoptosis-related proteins Bax,Bcl-2,Fas,FasL and TNF-α in the placental tissues were determined by means of immunohistochemical methods.It was showed that the apoptosis-related protein expressed both in villus and decidua of the placenta,most of which were expressed in syneytiotrophoblast（ST）.The positive cells with expression of Bax,Fas,FasL and TNF-α increased along with the increase of the pregnant days in both the experimental group and the control group,and the positive cells with expression of Bcl-2 decreased along with the increase of the pregnant days.It was also demonstrated that the positive cells with expression of Bax,Fas,FasL and TNF-α of the experimental group showed a higher percentage of expression than that of the control group on the same pregnant days,but the positive cells with Bcl-2 expression of the experimental group were fewer than that of the control group.It is concluded that the expression of apoptosis-related protein Bax,Bcl-2,Fas,FasL and TNF-α in the placenta were altered when the pregnant mice were infected with Toxoplasma gondii during the second trimester,which may induce the apoptosis through the endogenic and ectogenic pathway.
出处
《中国人兽共患病学报》
CAS
CSCD
北大核心
2010年第1期57-61,共5页
Chinese Journal of Zoonoses
基金
山西省科技厅自然基金项目(2009)
山西省高校科技研究开发项目(200613015)
山西医科大学创新基金(2009)
关键词
刚地弓形虫
免疫组织化学
胎盘
凋亡相关蛋白
Toxoplasma gondii immunohistochemistry placenta apoptosis-related protein
