摘要
石蜡切片是观察植物组织构造常用的制片方法之一。活的细胞或组织多为无色透明,各种组织间和细胞内各种结构之间均缺乏反差,在一般光镜下不易清楚区别;组织离开机体后很快就会死亡和产生组织腐败,失去原有正常结构,因此,组织要经固定、石蜡包埋、切片及染色等步骤以免细胞组织死亡,才能清晰辨认其形态结构。重点研究了植物石蜡切片的固定与保存,拟为今后固定剂及固定方法的选择提供科学依据。
paraffin section is one of the methods commonly used in observation of plant tissue. Living cells or tissues are most- ly colorless, transparent, and cells among the various organizations within the lack of contrast between the various structures, Under normal light microscope, not easy to clearly distinguish The organization will die soon after leaving the body and pro- duce the organization of corruption, loss of normal structure of the original, therefore, the organization must be subject to fixed, embedded in paraffin, sliced and stained tissue death and other steps to avoid, but can clearly identify their morpho- logical structure. This paper focuses on fixed paraffin sections of plant and preservation for future fixed - agents and a fixed method of providing a scientific basis.
出处
《安徽农学通报》
2010年第1期198-200,共3页
Anhui Agricultural Science Bulletin
关键词
石蜡切片
固定
保存
Paraffin sections
fixed
save
