摘要
目的:评价Real-time PCR技术结合浓缩涂片染色法对结核病快速诊断的应用价值。方法:应用Real-timePCR技术结合浓缩涂片染色法组合测定1 052份临床标本,并与直接涂片法、BACTEC MGIT-960快速培养法结果进行比较。结果:Real-time PCR技术结合浓缩涂片染色法的阳性检出率30.6%,显著高于直接涂片法的19.2%(χ2=36.6,P<0.01),而接近于BACTEC-960培养法的34.9%(χ2=2.5,P>0.05),而且检测时间明显缩短为4 h;以BACTECMGIT-960培养鉴定结果为标准评价,Real-time PCR技术结合浓缩涂片染色法的敏感性为89.7%(280/312),特异性为94.3%(698/740),符合率为93.0%(978/1 052)。结论:Real-time PCR技术结合浓缩涂片染色法用于结核分枝杆菌检测快速简便,具有很高的敏感性和特异性,在结核病早期诊断中有重要的临床价值。
Objective：To evaluat the applicative value of applying Real time PCR technology and concentration smear staining method to rapid diagnosing tuberculosis. Methods ：To apply Real-time PCR technology and concentration smear staining method to check 1 052 pieces of clinic specimen,and compare the result with those of direct smear method and BACTEC MGIT-960 rapid cultivation method. Results：The positive relevance ratio of Real-time PCR technology combining with concentration smear staining method was 30.6%, which was evidently higher than that of direct smear method of 19.2 % （x2= 36.6 ,P〈0.01） and closes to BACTEC-960 cultivation method of 34.9 % （x2 = 2.5 ,P〉0.05）. Moreover, the testing time was evidently shortened as 4 hours. At the standard of evaluation method of BACTEC MGIT-960 cultivation result, the sensitivity of Real-time PCR technology combining with concentration smear staining method was 89.7% （280/312）, the specificity was 94.3% （698/740） and the agreement was 93.0% （978/1 052）. Conclusion ：It is convenient andquick to apply Real-time PCR technology combining with concentration smear staining method to checking Myco-bacteria tuberculosis. It has very high sensitivity and specificity. It is important to apply to the clinic early diagnosing tuberculosis.
出处
《中国误诊学杂志》
CAS
2010年第1期7-9,共3页
Chinese Journal of Misdiagnostics
关键词
聚合酶链反应/方法
结核/诊断
染色与标记
Polymerase Chain Reaction/methods
Tuberculosis/diagnosis
Staining and Labeling
