摘要
目的:评价微弧氧化(MAO)及聚左旋乳酸(PLLA)封孔表面修饰对生物可降解WE42镁合金材料的生物相容性的影响。方法:以微弧氧化法制备陶瓷膜表面的WE42镁合金材料(MAO/WE42),再在陶瓷膜膜层表面进行PLLA封孔处理,制成MAO+PLLA修饰WE42镁合金材料(MAO+PLLA/WE42);扫描电镜观测材料在体外生理环境中的表面微观形貌;用材料浸提液处理人脐静脉内皮细胞,MTT比色法检测细胞相对增殖率(RGR),判断细胞毒性的级别;同时测定与材料表面接触2min后血浆的凝血时间(PT、RT)及经材料浸提液处理的2%人血悬液的溶血率(hemolytic ratio)。结果:扫描电镜观察显示WE42浸泡后出现严重腐蚀,MAO/WE42和MAO+PLLA/WE42材料浸泡前后变化较轻,未出现明显的腐蚀孔洞;WE42细胞毒性较强,而MAO/WE42、MAO+PLLA/WE42无明显细胞毒性作用;MAO/WE42、MAO+PLLA/WE42具有一定的抗凝血性能;溶血试验结果显示,MAO/WE42和MAO+PLLA/WE42的溶血率均低于WE42,且MAO+PLLA的抗溶血性能优于MAO(P<0.01)。结论:微弧氧化表面修饰可改善WE42的生物相容性,PLLA封孔处理可提高WE42的抗溶血性能,MAO+PLLA作为可控吸收镁合金药物洗脱支架的载药层是安全的。
Objective：To evaluate the effects of micro-arc oxidation （MAO） and poly-L-lactic acid （PLLA） surface-modification on the biocompatibility of magnesium alloy WE42. Methods：Micro-arc oxidation surface-modificated WE42 （MAO/WE42） materials and MAO＋PLLA surface-modificated WE42 （MAO＋PLLA/WE42） materials were prepared. The surface topography of materials in the physiological environment was observed in vitro by scanning electron microscopy. The human umbilical vein endothelial cells （HUVECs） were treated by leaching liquor of materials. The relative growth rate （RGR） was assessed by MTT assay to evaluate the level of cytotoxicity. The coagulation time（PT,RT） and hemolytic ratio were determined to evaluate the blood compatibility of those materials. Results：The observation of scanning electron microscopy indicated that WE42 corroded seriously after immersion. There were no obvious corrosion holes on the surface of MAO/WE42 and MAO＋ PLLA/WE42 materials after immersion. It was found that there were good anticoagulant properties but no apparent cytotoxicity in MAO/WE42 and MAO＋PLLA/WE42 materials. The results of hemolysis test showed that there were lower hemolysis rates in MAO and MAO＋PLLA materials than that of WE42. The material of MAO＋PLLA possessed the better anti-hemolytic properties than that of MAO （P〈0.01）. Conclusion：Micro-arc oxidation surface modification improved the biocompatibility of WE42. PLLA sealing treatment improved the anti-hemolytic performance of WE42. MAO＋PLLA provided a safe drug-loading surface for drug-eluting stents of magnesium alloy.
出处
《天津医药》
CAS
北大核心
2010年第1期26-28,81,共4页
Tianjin Medical Journal
基金
天津市应用基础及前沿技术研究计划重点项目(项目编号:08JCZDJC17900)
关键词
合金
镁
生物相容性材料
材料试验
显微镜检查
电子
扫描
乳酸
脐静脉
内皮细胞
alloys magnesium biocompatible materials materials testing microscopy electron scanning lactic acid umbilical veins endothelial cells
