摘要
目的:探讨早期应用生态免疫肠内营养对重症急性胰腺炎大鼠炎症反应和肠屏障损害的影响。方法:60只SD大鼠随机分为对照组(C组)、传统肠内营养组(EN组)和生态免疫型组(EIN组),每组20只。采用5%牛磺胆酸钠逆行注射法建立重症急性胰腺炎模型。于第12、24、48、72小时处死大鼠留取标本,检测血清巨噬细胞移动抑制因子(MIF),观察小肠组织病理学改变及运用免疫组化法和RT-PCR方法检测小肠组织中MIF的表达、Toll样受体4(TLR4)mRNA的相对含量。结果:(1)建模12h,EN组和EIN组MIF水平较C组明显升高(P<0.01);建模72h,3组的指标呈不同程度下降趋势,其中EIN组明显低于EN组(P<0.05)。(2)C组大鼠小肠组织MIF表达弱,EIN组和EN组阳性细胞数增加,EIN组较EN组阳性表达率低(P<0.05)。病理学观察显示72hEIN组病变程度较EN组轻。(3)大鼠给予肠内营养干预后72h,EIN组TLR4mRNA的相对含量较EN组显著降低(P<0.01)。结论:生态免疫型肠内营养能缓解重症急性胰腺炎大鼠炎症反应,减轻肠道屏障损害,缩短病程。
Objectives To investigate the effect of ecoimmunonutrition therapy on inflammatory response and gut mucosal barrier dysfunction in rats with early phase of severe acute pancreatitis （SAP）. Methods Sixty SD rats were divided randomly into control group （C group,n= 20）,traditional enteral nutrition group （EN group,n = 20） and ecoimmunonutrition group （EIN group,n = 20）. The SAP modles were induced in rats by retrograde injection of 5% deoxycholate into bilio-pancreatic duct. All the rats were killed at 12,24,48 and 72 h after modeling, macrophage migration inhibition factor （MIF） level was measured in serum. Observed the histopathological changes and also the MIF were determined by immunohistochemistry in small intestine. The expression of TLR4 mRNA in the pancreatic tissues were detected by reverse transcription polymerase chain reaction （RT-PCR）. Results （ 1 ） 12 h after modeling, serum MIF level was increased higher in EN group and EIN group than C group（P 〈 0.01 ）. At 72 h, MIF level was decreased on varying degrees among the three groups, the EIN group was significantly lower than the EN group （P 〈 0.05）. （2） Small intestine tissues of rats in C group have weak expression of MIF, while the number of positive cells increased markedly in EN and EIN groups. The positive expression rate of MIF was different between EN group and EIN group, in EIN group it was lower than EN group （P 〈 0.05）. The degree of pathological changes was lighter in EIN group than in EN group at 72 h. （3） Compared with C group, TLR4 mRNA level markedly increased after modeling 12 h in small intestine in EIN and EN groups （P 〈 0.01 ）, and the level of TLR4 mRNA was significantly lower in EIN group than in EN group after ecoimmunonutrition therapy was given （P 〈 0.01）. Conclusion Ecoimmunonutrition can be effectively regulated inflammatory reaction in rats of SAP, mitigate the damage of gut mucosal barrier and shorten the course.
出处
《实用医学杂志》
CAS
北大核心
2010年第1期20-23,共4页
The Journal of Practical Medicine
基金
2008年安徽省临床医学重点学科新技术引进项目(编号:2008N017)
关键词
胰腺炎
急性坏死性
肠道营养
生态免疫营养
Pancreatitis, acute necrotizing
Enteral nutrition
Ecoimmunonutrition
