摘要
【目的】评价国家种质库长期保存的国内外栽培豌豆(Pisum sativum L.)资源的遗传多样性水平,揭示其遗传多样性、等位基因和群体结构差异,据此评估其重要程度及价值,为中国豌豆资源研究策略和方向的正确选择、国内外资源的充分发掘利用和深入研究提供理论依据。【方法】利用21对豌豆多态性SSR引物,对来自国外五大洲66个国家和中国28个省(区、市)的1984份栽培豌豆进行SSR标记遗传多样性和群体结构分析;采用Structure2.2软件完成资源群体结构剖析、推断参试材料的合理组群数、确定每份参试材料的适当群体划入及其相关参数计算;利用NTSYSpc2.2d软件估算其遗传距离,进行主成分分析(PCA)并绘制三维空间聚类图;采用PopgeneV1.32估算种质群的等位位点分布等参数,利用FstatV2.9.3.2进行种质群间遗传多样性差异显著性测验。【结果】通过对国、内外栽培豌豆资源群间SSR等位基因数(NA)、有效等位基因数(NE)、有效等位基因所占比重(NE/NA)、等位基因丰度(AR)、基因多样性指数(GD)、Shannon's信息指数(I)的比较,发现除等位基因数(NA)外,国内资源的其它5个遗传多样性指标全面高于国外资源。在21个SSR基因位点中,国内、外资源群在7个位点间存在等位基因种类的差异。群体结构分析将1984份世界栽培豌豆资源划分成三大组群。组群A包含96.49%的国外栽培豌豆参试资源,可代表典型的国外栽培豌豆资源类型;组群B的资源88.18%来源于陕西和内蒙古,可代表中国典型的春播区栽培豌豆资源类型;组群C的资源52.05%源于中国秋播区,47.44%源于中国春播区,可代表中国秋播区和除陕西、内蒙古外的春播区栽培豌豆资源类型。组群间遗传多样性差异达到显著水平。PCA作图分析也明确显示世界栽培豌豆资源群体中存在3个边界明显的资源富集区(基因库I、II、III),且与三大组群的群体结构分析
[Objective] Assessing the genetic diversity between Chinese and alien accessions of cultivated pea （Pisum sativum L.） sampled from National Gene Bank, analyzing their differentiation on allelic loci and population structure, the study try to evaluate the importance and value of these conserved genetic resources, to provide essential information for guidance on strategy and direction choices for future studies of pea genetic resources in China, and for the effective exploration and utilization of global pea genetic resources. [ Method] 1 984 cultivated pea accessions from 66 countries of five continents and 28 provinces of China were employed for SSR analysis using 21 polymorphic primer pairs to detect genetic diversity and population structure. The Structure 2.2 software was used for population structure detection, definition of real population number, genotype allocation to its real population, and calculation of related parameters. Calculation of genetic distance, PCA analyses, 3-dimensional PCA graph, was conducted and drawn by NTSYSpc 2.2d statistical package. Allelic statistics were carried out by Popgene VI.32. The significance test between groups of genotypes was carried out by Fstat 2.9.3.2 statistical package. [ Result ] Based on SSR markers, the observed number of alleles （NA）, the effective number of alleles （NE）, the ratio of NE/NA, the allelic richness （AR）, the gene diversity （GD） and the Shannon＇s information index （I） of pea germplasm resources from China and foreign countries were thoroughly compared. Except the observed number of alleles （NA）, the values of other parameters on genetic diversity detected from Chinese landraces were all higher than that from foreign germplasm resources. Among the 21 tested SSR loci, the difference between Chinese and foreign germplasm collections appeared in 7 SSR loci. The population structure analysis divided all the 1 984 tested genotypes into 3 populations （Pop A, Pop B and Pop C）. Pop A consisted of almost all
出处
《中国农业科学》
CAS
CSCD
北大核心
2010年第2期240-251,共12页
Scientia Agricultura Sinica
基金
现代农业产业技术体系建设专项(nycytx-18)
农业部作物种质资源保护项目(NB09-2130135-2-09)
