摘要
目的探讨低温氧合血+Celsior液微流量持续灌注对大鼠右心的保护效果。方法Wistar大鼠切取全心,随机分为3组,实验组的心脏以4℃氧合血+Celsior液微流量(1mL/h)持续灌注低温保存4h;对照组以4℃Celsior液微流量持续灌注低温保存4h;单纯冷保存组以4℃Celsior液单纯浸泡保存4h。保存后的鼠心用Langendorff装置灌注,切除右心耳,将一球囊放入右心室,测定右心室血流动力学指标;高效液相色谱仪测定ATP含量;光镜和电镜观察心肌组织的形态学改变。结果灌注30min时,实验组的右心室收缩压(RVSP)为(26.64±3.3)mmHg,右室舒张压(RVDP)为(19.07±3.23)mmHg,右心室压力微分(±dp/dt)为(752±77)mmHg/s;对照组的RVSP为(23.98±4.02)mmHg,RVDP为(11.31±2.46)mmHg,±dp/dt为(548±103)mmHg/s;单纯冷保存组的RVSP为(22.38±3.65)mmHg,RVDP为(9.43±2.44)mmHg,±dp/dt为(457±151)mmHg/s,实验组的血流动力学指标优于对照组和单纯冷保存组(P<0.05)。实验组心肌细胞的ATP含量为(1.225±0.316)μmol/L,对照组为(0.852±0.303)μmol/L,单纯冷冻保存组为(0.587±0.232)μmol/L,实验组ATP含量明显高于对照组和单纯冷保存组(P<0.05)。实验组的组织学损害轻于对照组和单纯冷保存组。结论低温氧合血+Celsior液微流量持续灌注对大鼠右心有保护效果。
Objective To study the effects of continuous hypothermal oxygenated blood with Celsior cardiac preservation solution （CPS） microperfusion on rat right heart preservation. Methods Wistar rats were randomly divided into 3 groups and the whole heart was obtained. The isolated rat hearts were continuously microperfused by oxygenated blood with Celsior at 4℃ in experimental group,those in control group were microperfused with Celsior solution and those in solution group were preserved with Celsior solution for 4 hours respectively. Right atrial resection was performed before insertion of a latex balloon through the atrium into the ventricle. After preservation, the hemodynamic indexes of rat heart were measured using Langendorff system, including right ventricular systolic pressure （RVSP） , right ventricular diastolic pressure （RVDP） and the rate of the right ventricular pressure change （ ±dp/dt） ; then the contents of adenosine triphosphate （ATP） were determined by high-performance liquid chromatography and myocardium structures were observed under an electric microscope or by H-E. Results After 30 min perfusion of Langendorff system, the RVSP, RVDP and ＋ dp/dt were （ 26. 64±3.32 ） mmHg, （29.07±3.23 ）mmHg and （ 752± 77 ） mmHg/s respectively in experimental group ; （ 23.98±4. 02 ） mmHg, （ 11.31±2. d6） mmHg and （548±103） mmHg/s respectively in control group; （22.38±3.65） mmHg, （9.43±2.44）mmHg and （457±151 ）mmHg/s respectively in solution group. The hemodynamic indexes in experimental group were superior to those in control and solution group （P 〈 0.05 ）. The contents of ATP in experimental, control and solution group were （1. 225±0. 316）, （0. 852±0. 303） and （0. 587±0. 232）μmol/L respectively. The contents of ATP in experimental group were significantly than those in control and solution group （P 〈 0.05 ）. The changes of myocardium uhrastructure in experimental group were milder than those in control and solution group. Co
出处
《哈尔滨医科大学学报》
CAS
北大核心
2009年第6期548-551,共4页
Journal of Harbin Medical University
关键词
心肌保护
心脏保存液
大鼠
myocardial preservation
cardiac preservation solution
rats
