摘要
目的:通过对穴位和非穴位皮肤一氧化氮(NO)含量及皮肤导电量的比较,探讨针刺在穴位和非穴位效应差异的机制。方法:①对20例提供了知情同意书的健康志愿受试者进行穴位及非穴位皮肤NO含量的检测。实验分为针刺组、非针刺组,每组10例。分别选取一侧手厥阴心包经的间使穴和足太阳膀胱经的承山穴作为针刺部位,同时分别选取针刺同侧经脉的郄门穴和承筋穴作为穴位点,穴位点旁开1.0～1.5cm处为非穴位点,对穴位点及非穴位点皮肤NO进行收集。针刺时,轻刺入每个穴位约5～10mm,手针操作以60次/min的频率,捻转行针2min,以得气,留针20min,每5min行针1次。采用微量透析法对NO进行收集,用化学发光法对NO含量进行检测分析。②对20例健康志愿受试者测试皮肤导电量。分组及针刺方法同①。分别对心包经的郄门穴和膀胱经的承筋穴及其旁开1.0～1.5cm处的非穴位进行测试。结果:①针刺组与非针刺组比较,针刺组穴位和非穴位NO含量均高于非针刺组(P<0.05);针刺组组内比较,穴位NO含量高于非穴位(P<0.05);非针刺组组内比较,穴位NO含量与非穴位之间差异无统计学意义(P>0.05)。②正常人体皮肤导电量,穴位高于非穴位(P<0.05)。针刺组与非针刺组比较有升高趋势。结论:针刺可使穴位NO含量及皮肤导电量升高。正常情况下穴位具有潜在的活跃性,但与非穴位比较差异不明显;在针刺作用下,穴位的活跃性有了明显表达,类似于穴位被激活,从而发挥治疗疾病的作用。
Objective To observe the effects of acupuncture at acupoints and non-acupoints on the subcutaneous nitric oxide （NO） content and skin electric conduction quantity. Methods A total of 40 healthy volunteers （half males and half females at ages of 25-45 years） signed informed consent for detection of NO （n = 20） contents and skin electric conduction quantity （electric current, EC, n = 20） of the acupeint and non-acupoint area. The two series of 20 subjects were equally randomized into acupuncture group and non-acupuncture group, respectively. Jianshi （PO 5）, Ohengshan （BL 57） were punctured with filiform needles, with the needles twirled for 2 rain and retained for 20 min. Dermal electric current were detected by using Dermatren （electrodermal screening device）, and NO contents in the microdialysis fluid collected from the subcutaneous tissues of Ximen （PC 4）, Chengjin （BL 56） and non-acupoints 1.0～1.5 cm lateral to PC 4 and BL 56 were detected by chemiluminescence technique. Resuits ① NO contents of PC 4, BL 56 and two non-acupoint regions in acupuncture group were all significantly higher than those of two acupoints and two non-acupoints in non-acupuncture group （P〈0.05）. In acupuncture group, the NO contents of both PC 4 and BL 56 were strikingly higher than those of two non-acupoints （P〈0. 05）. In non-acupuncture group there were no significant differences between PC 4 and non-acupoint and between BL 56 and non-acupoint （P〉0.05）. （② The skin electric current values of PC 4 and BL 56 were significantly higher than those of two corresponding non-acupoints in both acupuncture and non-acupuncture groups （P〈0.05）. Following acupuncture stimulation, an increasing tendency was found in the electric current in comparison with non-acupuncture group but without significance （P〉0.05）. Conclusion Acupuncture stimulation can upregulate dermal NO content and and the skin electric current of acupoint region was significantly higher than that of non-acupo
出处
《针刺研究》
CAS
CSCD
北大核心
2009年第6期383-386,392,共5页
Acupuncture Research
基金
国家自然科学基金资助项目(No.30772830)
关键词
一氧化氮含量
皮肤导电量
针刺
穴
非穴
Dermal NO content
Cutaneous electric current
Acupuncture
Acupoint
Non-acupoint
