摘要
目的对树鼩抓取和保定、采血、灌胃基本实验技术方法进行探讨,逐步规范树鼩实验技术。方法选用成年树鼩进行抓取和保定,分徒手法和器具法(自制捕捉保定袋),对130只树鼩采用尾静脉、股动(静)脉两种采血方法;采取人用8号胃管可对树鼩经口灌胃给药。结果所采用徒手、器具的方法抓取和保定树鼩均能有效地控制动物,不会发生动物死亡或很少逃逸;两种方法都顺利采集到所需血量,股动(静)脉单次最大采血量可达2 mL而不损伤动物;12只树鼩连续灌胃10 d,成功率100%。结论自制的捕捉保定袋经济实用,摸索的几种树鼩实验技术方法具有操作简便、安全、快捷等优点。
Objective To explore some practical methods to seize and fix, blood collection, and gastric lavage in tree shrews, and make efforts to develop standardized experimental techniques. Methods 130 adult tree shrews were hold and fixed by hand or with a cloth bag device. Blood samples were collected from the tail vein or puncture in the femoral artery （vein） with a syringe needle. Size 8 human gastric tube was used to make gastric feeding per os in the tree shrews. Results All tree shrews were effectively and well controlled by hand seizing or bag fixing, and there was no unexpected death or few escape. Blood collection was smoothly completed in all tree shrews by both methods and the largest bh）od volume was 2 mL taken from the femoral artery without injury to the animals. Gastrogavage into stomach was fully succeeded in 12 tree shrews for consecutive 10 days, with a success rate of 100% . Conclusion The bag fixing device is economieal and practical. The above described experimental techniques are simple, safe, practical, rapid and easy methods to use on tree shrews.
出处
《中国比较医学杂志》
CAS
2009年第12期76-78,I0004,共4页
Chinese Journal of Comparative Medicine
基金
云南省科技基础条件平台建设计划(2006PT07-2)
关键词
树[鼠句]
抓取
保定
采血
灌胃
Tree shrew
Seize
Fix
Blood collectiun
Gastric lavage
