摘要
目的探讨尿中Ⅳ型胶原在肾小球疾病诊断中的应用价值。方法应用竞争抑制性酶联免疫吸附实验，检测了２０例健康人及３８例肾小球疾病患者尿中Ⅳ型胶原的水平，并与肾活检的组织病理进行了比较。结果病理改变较为严重的患者以及肾活检为系膜增生性肾小球肾炎并伴有局灶性硬化、粘连和（或）新月体形成的患者，尿中Ⅳ型胶原的含量明显高于正常对照；但肾活检仅显示系膜增生性肾小球肾炎的患者，尿中Ⅳ型胶原水平同正常对照。结论尿中Ⅳ型胶原水平的测定，可以作为一个无创性的临床检测指标，有助于肾小球疾病的病理变化及其程度的诊断。
Objective〓To explore the value of detecting urinary type Ⅳ collagen in diagnosis of glomerular diseases. Methods〓Urinary type Ⅳ collagen was measured by using the competitive enzyme linked immunosorbent assay (ELISA) in 20 healthy controls and 38 patients with glomerular diseases, and the results were compared with pathologic changes revealed by renal biopsies. Results〓Urinary type Ⅳ collagen increased significantly in patients with severe pathologic changes in glomeruli as well as patients with mesangial proliferative glomerulonephritis which accompanied by focal glomerulosclerosis, synechiae and (or) cresents, while the urinary type Ⅳ collagen in patients with mesangial proliferative glomerulonephritis was at the same level as normal controls. Conclusion〓The measurement of urinary type Ⅳ collagen, as a noninvasive clinical marker, is useful in diagnosis and understanding pathologic changes and severity of glomerular diseases.
出处
《中华儿科杂志》
CAS
CSCD
北大核心
1998年第10期615-618,共4页
Chinese Journal of Pediatrics
关键词
胶原
细胞外基质
肾小球疾病
诊断
Collagen〓〓Extracellular matrix〓〓Glomerul onephritis, membranoproliferative
