摘要
本文用高效液相色谱(HPLC)结合生物鉴定分析了杨树(Ⅰ-69)枝条顶端营养芽从秋季(8月)到翌年春季(4月)内源激素活性及含量的变化。生长抑制物质的活性从8月至11月是不断增加的,11月以后逐渐降低。脱落酸的含量在11月最高,4月芽萌发时最低。生长促进物质的活性在12月之前一直很低,其后逐渐增加。亦霉素含量在11月之前变化不大,11月以后随着休眠的的解除,赤霉素有明显积累。结合态脱落酸和赤霉素的含量变化趋势与其游离态的含量变化趋势呈负的相关。温室切枝水培试验表明,外源脱落酸能维持芽的休眠,推迟萌发;而外源赤霉素能解除休眠,促进萌发。但它们对11月前所采的枝条都无明显效应。
Changes of endogenous hormone contents in terminal buds of poplar 1-69 from autumn to spring were determined by bioassay and HPLC. It was found that the activity of growth-inhibiting substances was increased in autumn and decreased in winter and spring. Content of ABA was much lower (65μg/kg F. W.) in April 8 than in Oct. 15(972μg/kg F. W.). Obvious increase in GA activity was observed during the release of bud dormancy. From Oct. 15 and thereafter, GA3 content increased gradually and reached its maximum (342μg/kg F. W.) by Apr. 8. Content of B-ABA and B-GA3 was negatively correlated to F-ABA and F-GA3 respectively.The experiment also showed that exogenous GA3 and GA4+7 were effectively in breaking the bud dormancy of poplar twigs in greenhouse. On the other hand, treatment with ABA delayed the ending of bud dormancy. However, exogenous hormones treatment did not affect the bud dormancy of poplar twigs if they were collected on Oct. 12. But dormancy of poplar seems to be regulated by the interaction between growth-promoting and growth-inhibiting substances.
出处
《南京林业大学学报（自然科学版）》
CAS
CSCD
1990年第1期7-15,共9页
Journal of Nanjing Forestry University：Natural Sciences Edition
