摘要
目的研究白血病细胞的多药耐药性（ＭＤＲ）。方法用ＲＴ－ＰＣＲ方法检测ＨＬ６０，ＨＬ６０／ＤＮＲＭＤＲ１的ｍＲＮＡ表达，用流式细胞仪分析单抗ＵＩＣ２标记的细胞株Ｐ糖蛋白（Ｐｇｐ）的表达，了解它们摄取和滞留荧光素Ｒ１２３的功能。结果ＨＬ６０／ＤＮＲ在ｍＲＮＡ水平高度表达ＭＤＲＩ，在蛋白质水平高度表达Ｐｇｐ，功能性试验中细胞内Ｒ１２３最终浓度ＨＬ６０为ＨＬ６０／ＤＮＲ的５０倍。
PURPOSE To study multidrug resistance (MDR) in leukemic cells. METHODS RT-PCR was used to detect the expression of MDR1 mRNA in HL60 and HL60/DNR. Flow cytometry analysis was used to detect the expression of P-glycoprotein (Pgp) in the cell lines labelled by monoclonal antibody UIC2, and to study these cell lines'uptake and retention of a Pgp substrate rhodamine 123 (R123). RESULTS The expression of MDR1 mRNA and Pgp in HL60/DNR are strong positive. The intracellular concentration after uptake and retention of R123 in HL60 is 50 times more than that in HL60/DNR. CONCLUSIONS HL60/DNR is a model cell line of MDR1.
出处
《上海医科大学学报》
CSCD
1998年第5期394-397,共4页
Journal of Fudan University(Medical Science)
