摘要
[目的]为脂联素基因作为脂肪沉积候选基因的研究提供依据。[方法]通过猪BAC文库筛选及primer-walking的测序方法获得脂联素基因的启动子序列,采用PCR-RFLP技术对蓝塘猪、大花白猪、大白猪、长白猪和杜洛克猪5个品种共290头猪的脂联素基因启动子区的多态性进行了分析。[结果]脂联素基因的5’侧翼区-1 010 bp(G/A)的SNP位点,本地猪种中GG基因型频率明显高于外来猪种;-394 bp(T/C)的SNP位点,本地猪种基因型分布较为丰富,而在外来猪种中却没有检测到CC基因型,且外来猪中T等位基因频率较高。[结论]脂联素基因上游-1 010 bp(G/A)的SNP位点突变可能会引起该基因转录水平的改变,而-394 bp(T/C)的SNP位点与基因转录水平及与脂肪沉积可能无关。
[ Objective ] The aim was to provide basis for study on adiponectin as candidate gene of fat deposition. [ Method ] By screening porcine BAC library and using primer-walking method, the promoter sequence of adiponectin was obtained. The polymorphisms of 290 pigs were analyzed with PCR-RFLP technology, including Lantang pig, Large spotted pig, Large white pig, Landrace and Duroc. [ Result] At SNP site of adiponectin 5＇ -flanking region - 1 010 bp（ G/A）, GG genotype frequency in Chinese indigenous pigs was significantly higher than in exotic pigs; at SNP site of adiponectin 5＇ -flanking region -394 bp （T/C） , the genotype distribution in Chinese indigenous pigs was abundant, while in exotic pig, CC genotype was Porcine BAC library; Adiponectin; Promoter; SNPnot detected and T allele frequency was higher. [ Conclusion] SNP site mutation of - 1 010 bp（G/A） may lead to changes of the gene transcription level, while SNP site of -394 bp（T/C） may have no relation with gene transcription level and fat deposition.
出处
《安徽农业科学》
CAS
北大核心
2010年第1期413-415,418,共4页
Journal of Anhui Agricultural Sciences
基金
农业部"948"项目资助(2006-G50)
