摘要
以小水榕根尖为材料,通过取材时间,8-羟基喹啉、饱和对二氯苯2种药剂预处理,并以细胞中有丝分裂相的数目、染色体的清晰度、分散程度为指标,探索出适于小水榕染色体分析的方法。结果表明:小水榕有丝分裂中期在一天的16:00达到高峰期,取样时间确定在16:00。饱和对二氯苯水溶液预处理2 h,卡诺氏固定液固定2 h,1mol/L盐酸60℃水浴中解离3 min～4 min,并以醋酸洋红染色效果较好。小水榕核型为2 n=2 x=30=12 m+14 sm+4 st,属于"2B"类型。
The purpose of this work was to provide the approprtate method of chromosome analysis using the root-tip of Anubias barteri as materials. The key factors, such as the sampling times, pretreatment agents （8-hydroxyquinoline and saturated p-dichlorobenzene）, and methods which influences the preparation of chromosome were considered. Based on the contrasts of numbers of mitosis metaphase cells, the degree of treatment complications and the fact that chromosomes were distinct and countable from their background under microscope, the suitable root-tip squashing method for chromosome preparation of Anubias barteri were as follows： sampling materials at 16：00 pm and fixating with Carnoy＇ s I, pretreating with saturated p-dichlorobenzene for 2 h, dissociating with 1 mol/L HC1 at 60 ℃ for 3 min - 4 min, and staining with acetocarmine. The karyotype ofAnubias barteri were as follows：2 n =2 x =30 = 12 m ＋ 14 sm ＋4 st, belonging to ＂2B＂ of Stebbins.
出处
《激光生物学报》
CAS
CSCD
2009年第6期782-785,793,共5页
Acta Laser Biology Sinica
基金
安徽省教育厅"十五"学术带头人基金项目(2002年度)
关键词
小水榕
核型分析
染色体
Anubias barteri
karyotype analysis
chromosome
