摘要
目的探讨哮喘患者CD4+ CD25+ T调节细胞的作用是否存在缺陷及药物(地塞米松、小剂量氨茶碱)对其作用的影响,为有效控制哮喘提供依据。方法分离哮喘患者及健康对照者外周血CD4+ CD25+ T调节细胞及CD4+CD25-T淋巴细胞并培养,设CD4+ CD25+ T调节细胞组、CD4+CD25-T淋巴细胞组及CD4+ CD25+ T调节细胞联合CD4+CD25-T淋巴细胞组,第3组又分为氨茶碱干预组、地塞米松干预组及空白对照组,72h后取培养上清液用ELISA法检测IFN-γ、IL-5、IL-13水平。结果健康对照者CD4+ CD25+ T调节细胞可抑制IFN-γ、IL-5及IL-13的生成(P<0.01),哮喘患者仅抑制IFN-γ、IL-5的生成(P<0.01);地塞米松预处理CD4+ CD25+ T调节细胞后,健康对照者CD4+ CD25+ T调节细胞抑制IFN-γ(P<0.05)、IL-5(P<0.01)、IL-13(P<0.01)生成能力增强,哮喘患者抑制IL-5、IL-13生成能力增强(P<0.01);采用氨茶碱预处理后,健康对照者CD4+ CD25+ T调节细胞抑制IL-5(P<0.01)、IL-13(P<0.05)生成能力增强,哮喘者抑制IL-5的能力增强(P<0.01)。结论哮喘患者CD4+ CD25+ T调节细胞在抑制Th2型细胞因子产生方面存在部分功能缺陷;地塞米松可增强健康者和哮喘者CD4+ CD25+ T调节细胞抑制Th2型细胞因子的能力;氨茶碱可增强健康者CD4+ CD25+ T调节细胞抑制Th2型细胞因子的能力,部分增强哮喘者CD4+ CD25+ T调节细胞的该抑制能力。
Objective To investigate whether the CD4 ＋CD25＋ regulatory T cells in asthmatic patients act insufficiently and how dexamethasone and low dose of aminophylline affect the CD4 ＋CD25＋ T cells. Methods CD4 ＋CD25＋ and CD4 ＋CD25＋T cells were separated from healthy controls and asthmatic patients,then cultured as CD4＋CD25＋ T cell group,CD4＋CD25 T cell group and CD4 ＋CD25＋-CD4＋CD25t T cell group,the last group was setted three sub groups as dexamethasone group,aminophylline group and blank control. After 72h,the supernatant was collected and IFN-γ,1L-5 and IL- 13 levels were detected by ELISA. Results The CD4＋ CD25＋ regulatory T cells from healthy controls could inhibit the IFN-γ,IL-5 and IL-13 levels（P〈0.01） ,while CD4＋ CD25＋ regulatory T cells from asthmatic patients could inhibit the IFN 7 and IL-5 levels （P〈0. 01）. After exposure of CD4＋ CD25 ＋ regulatory T cells to dexamethasone, the regulatory cells＇ inhibit effects on IFN-γ,IL -5 and IL-13 were enhanced in healthy controls, while in asthmatic patients, the inhibit effects on IFN-γand IL-5 were enhanced. After exposure of CD4＋ CD25＋ regulatory T cells to low dose of aminophylline, the regulatory cells＇ inhibit effects on IL- 5 and IL-13 were enhanced in healthy controls, while in asthmatic patients,the inhibit effect on IL-5 was enhanced. Conclusion CD4＋ CD25＋ regulatory T cells from asthmatic patients act insufficiently on inhibiting the Th2 cytokines,dexamethasone can enhance both healthy and asthmatic original CD4＋ CD25＋ regulatory T cells＇ inhibition on Th2 cytokines,aminophylline can enhance the inhibition of CD4＋ CD25＋ regulatory T cells on Th2 cy tokines in healthy controls,while in asthmatic patients, the inhibition is partly enhanced.
出处
《重庆医学》
CAS
CSCD
北大核心
2010年第1期43-45,47,共4页
Chongqing medicine
