摘要
目的研究慢性阻塞性肺疾病(COPD)患者支气管黏膜中过氧化物酶增殖物激活受体-γ(PPAR-γ)、基质金属蛋白酶-9(MMP-9)和诱生型一氧化氮合酶(iNOS)的表达及与气流阻塞的关系。方法根据吸烟史及肺功能检查将其受试者分为3组:吸烟伴COPD患者18例;吸烟肺功能正常组15例;不吸烟肺功能正常组15例。全部受试者经电子支气管镜取支气管黏膜标本,采用免疫组织化学法检测支气管黏膜中核转录因子PPAR-γ、MMP-9和iNOS的表达,并进行相关性分析。结果①免疫组织化学染色结果:核转录因子PPAR-γ在吸烟伴COPD组和吸烟肺功能正常组表达明显减少,MMP-9和iNOS在两组表达明显增多,与不吸烟肺功能正常组比较差异均有统计学意义(P<0.01);②相关性分析:核转录因子PPAR-γ的表达与FEV1%、FEV1/FVC%呈直线正相关(P<0.01);MMP-9和iNOS的表达与肺功能指标呈直线负相关(P<0.01)。COPD患者支气管黏膜中核转录因子PPAR-γ的表达与MMP-9和iNOS的表达均呈直线负相关(P<0.05)。结论COPD患者支气管黏膜中核转录因子PPAR-γ表达减少导致气道的抗炎能力下降,气道内炎症细胞及炎症介质增多,导致肺组织损伤及重构,引起和加重COPD患者的气流阻塞。
Objective To investigate expressions of PPAR-γ matrix metalloproteinase-9（MMP-9）and induced nitric oxide synthase（iNOS）in the bronchial mucosa of patients with chronic obstructive pulmonary disease（COPD）and to explore their correlation with airflow obstruction.Methods：Based on their smoking history and lung function,they were divided into three groups：18 smokers with COPD,15 smokers and 15 non-smokers with normal lung function.Expressions of PPAR-γ,MMP-9 and iNOS in the bronchial mucosa were determined by immunohistochemistry.Results ① Immunohistochemical staining showed that PPAR-γ was decreased in smokers of the COPD group and smokers of the normal lung function group,while MMP-9 and iNOS were increased in both groups compared with nonsmokers with normal lung function（P〈0.01）.② The bronchial mucosa levels of PPAR-γ was positively correlated with FEV1% and FEV1/FVC%（P〈0.01）,however MMP-9 and iNOS was inversely correlated with FEV1% and FEV1/FVC%（P〈0.01）.Expression of PPAR-γ was inversely correlated with MMP-9 and iNOS（P〈0.05）.Conclusions The decrease of PPAR-γ in bronchial mucosa of patients with COPD may lead to abnormal inflammation of the airway,and thus produces or aggravates airway obstruction in patients with COPD.
出处
《山东大学学报（医学版）》
CAS
北大核心
2009年第12期91-94,共4页
Journal of Shandong University:Health Sciences
基金
山东省自然科学基金资助项目(Y2001C01)
山东省医药卫生科技发展计划资助项目(HZ059)
