摘要
目的:检测过氧化物酶体增殖物激活受体(Peroxisome proliferator-activated receptorγ,PPAR-γ)在大鼠心肌梗死时心肌细胞中的表达水平,探讨厄贝沙坦逆转心室重塑的机制是否与PPAR-γ有关。方法:结扎大鼠冠状动脉前降支建立心肌梗死模型,24h后存活大鼠随机分成心肌梗死(Myocardial infarction,MI)组、厄贝沙坦(Irbesartan,Ire)组75mg(/kg·d),另设假手术(Sham)组。6周后测定PPAR-γmRNA及蛋白的表达情况,左室重量指数,心肌细胞横切面积,测定心肌胶原容积分数。结果:与假手术组比较,MI组大鼠心肌中PPAR-γmRNA及蛋白表达水平显著降低,左室重量指数增加,心肌细胞横截面积增加,非梗死区胶原沉积明显,胶原比例增加。Ire组心肌中PPAR-γmRNA及蛋白表达水平增高,左室重量指数降低,心肌细胞横截面积减少,非梗死区胶原沉积减少。结论:PPAR-γ在大鼠心肌梗死时心肌细胞中的表达下调;厄贝沙坦可能通过上调并激活PPAR-γ逆转心肌梗死后左室重塑。
Objective：To study the expreesion of PPAR-γ in the left ventricular myocardium in rats with myocardial infarction ,and to investiagate whether the mechanism of Irbesartan reversing the ventricular remodeling is related to PPAR-γ. Methods：Myocardial infarction models were successfully induced by ligation of anterior descending coronary artery.24 h later the survivors were randomly divided into 3 groups.① MI group;② Irbesartan group 75 mg/（kg·d）;③ The rats with sham ligation formed Sham group.After 6 weeks, the expreesion of PPAR- γ , the ratio of LV weight to body weight （ LVWI ）, cross-section area （ CSA ）of left ventricular myocardium were examined.The collagen volume fraction （CVF） were analyzed by PSR dyeing. Results：In MI group, compared with those in Sham group, the expreesion of PPAR-γ mRNA and protein in the left ventricular myocardium decraced significantly, but the LVWI, CSA and the CVF in non-infarcted zone increased.Compared with those in MI group,in Ire group,the expreesion of PPAR-γ mRNA and protein in cadiocyte incraced , while the LVWI ,CSA and the CVF in non-infarcted zone decreased.Conculusion： The expression of PPAR-γ is down-regulated in the left ventricular myocardium with myocardial infarction, Irbesartan reverses the left ventricular remodeling possibly by up-regulating arid activating PPAR-γ.
出处
《重庆医科大学学报》
CAS
CSCD
北大核心
2009年第12期1670-1672,共3页
Journal of Chongqing Medical University
