摘要
目的探讨趋化因子受体CXCR4在胃癌和腹腔内转移灶中的表达及其意义。方法免疫组化法检测胃癌原发灶、淋巴转移灶、腹膜转移灶以及正常胃黏膜组织中的CXCR4表达情况。RT-PCR半定量检测胃癌原发灶、淋巴转移灶、腹膜转移灶以及正常胃黏膜组织中CXCR4 mRNA表达水平。结果免疫组化结果显示CXCR4在胃癌原发灶中的表达与肿瘤的分期、浸润深度及淋巴结转移有关(P<0.05)。RT-PCR结果显示早期胃癌中,进展期胃癌组织、淋巴结转移灶和腹膜转移灶中的CX-CR4 mRNA表达呈依次增强,各分组间CXCR4的表达差异有显著性(P<0.05)。结论CXCR4在原发灶中的表达与胃癌分期、肿瘤的浸润深度及淋巴结转移有关。
Objective To study the expresion and significance of CXCR4 in gastric cancer（GC） and intra-abdominal metastasis.Methods Immunohistochemistry and PT-PCR were used to detecte the expresion of CXCR4 protein and CXCR4 mRNA in GC,lynph node metastasis（LNM）,peritoneal metastasis（PM） and normal stomach（NM） tissues.Results There was no CXCR4 expression in normal gastric mucosa cell.The expression rate of CXCR4 in GC and PM was 59.4%,100% respectively（P〈0.05）.The expression of CXCR4 in primary tumor was correlated with tumor stage,depth of tumor invasion and lymph node metastasis（P〈0.05）,but not with age,gender,location of tumor,tumor size and pathological differentiation of patients（P〈0.05）.（2）Semi-quantatitive RT-PCR analysis showed that there was no CXCR4mRNA expression in normal gastric mucosa.Low quantity of CXCR4mRNA was detected in 5 early cancer samples,high quantity of CXCR4mRNA was detected in specimens of all advanced cancer tissue;the quantity of CXCR4mRNA in LNM and PM was the highest（P〈0.05）.Conclusions CXCR4 is expressed in both primary and metastic lesion in gastric cancer.Moreover,CXCR4 is upregulated when the tumor cell metastasize to lymph node and peritoneum.CXCR4 expression in primary tumor is correlated with tumor stage,invasion depth,LNM and PM.As the cancer progress,the expression of CXCR4 is upregulated,which implies that tumor cell with high expression of CXCR4 have more aggressive and metastatic capacity.
出处
《中国普通外科杂志》
CAS
CSCD
北大核心
2009年第12期1275-1279,共5页
China Journal of General Surgery
