摘要
目的探讨抗氧化剂α-硫辛酸对急性胰腺炎（AP）的治疗作用以及可能的机制。方法3．5％牛磺胆酸钠逆行胰胆管注射制备AP大鼠模型，数字表法随机分为假手术组、AP组、生理盐水组和α-硫辛酸组，每组30只。α-硫辛酸组于造模后腹腔内注射α-硫辛酸1mg／kg体重，生理盐水组注射等量生理盐水。分别于术后1、3、6、9、12h处死大鼠，检测血清淀粉酶、TNF-α、ICAM-1水平，观察胰腺病理改变，测定胰腺组织超氧化物歧化酶（SOD）活力、丙二醛（MDA）含量。结果AP组胰腺水肿、粘连、坏死，腹腔内可见血性腹水。术后6hAP组血清淀粉酶、TNF-α、ICAM-1水平以及胰腺组织MDA含量分别为（2211．0±547．4）U／L、（174．8±7．9）ng／ml、（49．3±8．0）ng／ml和（32．2±5．9）U／mgprot，较假手术组的（160±23）U／L、（6．5±1．1）mg／ml、（13．9±3．4）mg／ml、（16．2±3．2）U／mgprot明显升高（P〈0．05）；胰腺组织SOD活力为（38．5±9．5）U／mgprot，显著低于假手术组（56．7±6．6）U／mgprot（P〈0．05）。α-硫辛酸6h组的血清淀粉酶、TNF-α、ICAM-1水平以及胰腺组织MDA含量分别为（1478±642）U／L、（164．8±6．2）ng／ml、（37．5±3．9）ng／ml和（20．2±8．4）U／mgprot，较AP组显著降低（P〈0．05）；胰腺组织SOD活力为（66．0±8．6）U／mgprot，较AP组显著升高（P〈0．05）。结论AP发病与氧化应激有关，抗氧化剂α-硫辛酸对AP大鼠具有较好的治疗作用，其机制可能与抑制TNF-α、ICAM-1活性有关。
Objective To investigate the protective effects of α-lipoic acid in rats with acute pancreatitis （AP） and its potential mechanism. Methods Wistar rats were randomly divided into four groups according to random number table： sham operation （SO） group, AP group, normal saline （NS） group and α- lipoie acid group with 30 rats in each group. AP model was induced by retrograde injection of 3.5% sodium taurocholatc into the pancreatobiliary duct. Rats in α-lipoic acid group immediately received α-lipoic acid intra peritoneal injection at the dose of l mg/kg. Rats in NS group received same amount of normal saline, The rats were sacrificed at 1 , 3, 6, 9 and 12 h after AP induction. The serum levels of amylase, TNF-α and ICAM-1 were measured. Pancreatic histological changes were observed. The activities of pancreatic SOD and MDA were measured. Results In rats of AP group, optical microscopy showed pancreatic edema, adhesion and necrosis. The serum amylase, TNF-α, ICAM-1 and MDA levels in pancreatic tissue 6h after operation were （2211 ± 547） U/L, （ 174.8 ± 7, 9 ） ng/ml, （49.3 ± 8.0 ） ng/ml and （ 32.2 ± 5.9 ） U/mg prot, respectively, in AP group ; which were significantly increased when compared with those of SO group （P 〈 0.05 ）. Pancreatic SOD activity was （ 38.5 ± 9.5 ） U/mg prot, which was significantly lower than （ 56.7 ± 6.7 ） U/nag prot of SO group（P 〈0.05）. The serum amylase, TNF-α, ICAM-1 and MDA levels in pancreatic tissue 6 h after operation in α-lipoic acid group were （ 1478 ± 642 ） U/L, （ 164.8 ± 6.2） ng/ml, （37.5 ± 3.9） ng/ml and （20.2 ± 8.4） U/rag prot, respectively; which were significantly decreased when compared with those of AP group （P 〈 0. 05 ）. Pancreatic SOD activity was （66.0±8.6）U/mg prot, which were significantly higher than （38.5 ±9.5）U/rag prot of AP group （P 〈 0. 05）. Conclusions The pathogenesis of AP was associated with oxidative stress, and α-lipoic acid as an antioxidant played a role
出处
《中华胰腺病杂志》
CAS
2009年第6期406-409,共4页
Chinese Journal of Pancreatology
关键词
胰腺炎
抗氧化剂
硫辛酸
超氧化物歧化酶
丙二醛
Pancreatitis
Antioxidants
Thioctic acid
Superoxide dismutase
Malondialdehyde
